RESUMEN

Two-sector linked-scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60,000 Da) has allowed the discovery and assignment of an amino-terminal post-translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked-scan methods for the analysis of complex biopolymer mixtures.

Asunto(s)

Piruvato Descarboxilasa/genética , Secuencia de Aminoácidos , Secuencia de Bases , Datos de Secuencia Molecular