RESUMEN

Molecular characterization of seven Diocleinae lectins was assessed by sequence analysis, determination of molecular masses by mass spectrometry, and analytical ultracentrifugation equilibrium sedimentation. The lectins show distinct pH-dependent dimer-tetramer equilibria, which we hypothesize are due to small primary structure differences at key positions. Lectins from Dioclea guianensis, Dioclea virgata, and Cratylia floribunda seeds have been crystallized and preliminary X-ray diffraction analyses are reported.

Asunto(s)

Fabaceae/química , Lectinas/química , Plantas Medicinales , Secuencia de Aminoácidos , Cristalización , Concentración de Iones de Hidrógeno , Lectinas/aislamiento & purificación , Espectrometría de Masas , Datos de Secuencia Molecular , Peso Molecular , Lectinas de Plantas , Semillas/química , Alineación de Secuencia , Relación Estructura-Actividad , Difracción de Rayos X