RESUMEN
Lectins were extracted from the surface of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 and from its mutant A. brasilense Sp7.2.3 defective in lectin activity. The ability oflectins to stimulate the rapid formation of hydrogen peroxide related to increase of oxalate oxidase and peroxidase activity in the roots of wheat seedlings has been demonstrated. The most rapid induced pathway of hydrogen peroxide formation in the roots of wheat seedlings was the oxalic acid oxidation by oxalate oxidase which is the effect oflectin in under 10 min in a concentration of 10 microg/ml. The obtained results show that lectins from Azospirillum are capable of inducing the adaptation processes in the roots of wheat seedlings.
Asunto(s)
Azospirillum brasilense/metabolismo , Proteínas Bacterianas/metabolismo , Lectinas/metabolismo , Oxidorreductasas/biosíntesis , Peroxidasa/biosíntesis , Proteínas de Plantas/biosíntesis , Raíces de Plantas/enzimología , Triticum/enzimología , Proteínas Bacterianas/farmacología , Inducción Enzimática/efectos de los fármacos , Inducción Enzimática/fisiología , Lectinas/farmacologíaRESUMEN
It was found that Azospirillum brasilensis strain Sp7 is able to produce extracellular proteolytic enzymes. The enzymes were active within a broad range of pH values, with two peaks of activity being located in the acid and alkaline pH areas; required calcium ions; and exhibited substrate specificity with respect to azogelatin. Zymography allowed at least four proteolytic enzymes with molecular weights of 32, 45, 52, and 174 kDa to be detected in A. brasilense Sp7 culture liquid. It was shown that the lectin from A. brasilense Sp7 can inhibit proteolytic enzymes.