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Effects of disodium salt 2,4-di(1-metoxyethyl)-deuteroporphyrine-IX (Dimegin) and the light from Soret band (¼395-405 nm) at the viability of microbial cells and at their potential to form microbial biofilms have been compared with traditional antiseptics. Irradiation of microbial cells of S. aureus, E. coli, C. albicans and others with diode light (power density 0.05 Wt/cm2) caused a bactericidial effect similar to that obtained with standard anticeptics (chlorhexidine and dioxidine). A comparative study of the effectiveness of Dimegin and Photoditazine (a soluble salt of chlorine e6) as photosensitizers have been performed using the test system of erythrocyte hemolysis in vitro under irradiation with light from the Sore band. Results have shown insignificant difference in the photodynamic effect with similar doses of absorbed light and preparation concentration.

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AIM: Study the effect of 1270 nm wavelength laser exposure on the viability of Staphylococcus aureus and S. epidermidis cells in vitro. MATERIALS AND METHODS: Reference strain S. aureus 209P and clinical isolate S. epidermidis 26/193 that form bacterial biofilm were used in the study. Ica gene presence in S. epidermidis 26/193 that controls biofilm formation was confirmed in the study by PCR with a primer that generates a specific 814 n.p. amplicon. Experimental device by "New surgical technologies, Ltd." was used as a source of emission. The device has a continuous emission mode of laser semiconductor diodes with 1270 nm wavelength. Maximum regulated power of the emission is up to 3 W. Emission power in the studies performed was 150 mW. The time of exposition was 5, 10, 15 and 30 minutes. The amount of viable cells in the experiment and control was determined by calculating CFU/ml. Evaluation of bacterial biofilm formation was performed by the method described in O'Toole G.A. et al. (2000). RESULTS: A decrease of the number of viable forms of S. aureus 209P by a mean of 52 +/- 6.0% and 76 +/- 4.0%, and of S. epidermidis 26/193 by a mean of 48 +/- 4% and 64 +/- 5% for 15 and 30 min exposition, respectively, and a significant suppression of biofilm formation by staphylococci was shown. CONCLUSION: Exposure to 1270 nm laser renders a moderate bactericidal effect on staphylococci cells and significantly suppresses their bacterial biofilm formation.

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Accumulation of photosensibilisators - derivatives of E6 chlorines ("Radachlorine", "Photoditazine", "Zelevsky's balsam") in the mucous membrane and selection of most effective sources of emission have been investigated in 30 patients with rhinosinusitis and 10 with tonsillitis. As a source of emission we used light emitting diode (LED) matrix device "ACT" (wavelength approximately 405 nm (Sore band)) and a laser device LAHTA-"MILON"-ML500-SP (wavelength 662 nm). Drug accumulation in the mucous membrane and changes of their concentrations after emission were evaluated by changes of fluorescence, measured with a LESA-01-BIOSPEC spectrometer. The percent of fluorescence decrease ranged from 50% to 92.7%. This suggests intensive disintegration of photosensibilisators, and consequently, high therapeutic activity of this method. Effectiveness of this method is also confirmed by clinical results.

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In laboratory experiments, the rhizobacteria Azospirillum lipoferum 137, Arthrobacter mysorens 7, Agrobacterium radiobacter 10, and Flavobacterium sp. L30 were found to have a relatively high resistance to the toxic heavy metals lead and cadmium (except that strain L30 was found to be sensitive to Cd). When introduced by means of seed bacterization, the heavy metal-resistant strains actively colonized the rhizosphere of barley plants cultivated in uncontaminated and contaminated soils. In both pot and field experiments, seed bacterization improved the growth of barley plants and the uptake of nutrient elements from soil contaminated with Pb and Cd. The bacterization also prevented the accumulation of Pb and Cd in barley plants, thereby mitigating the toxic effect of these heavy metals on the plants.

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The capacities of laser biophotometry in the diagnosis of tuberculous spondylitis and its complications were studied in 42 patients with this disease and in controls. There was a definite significant trend of changes in the reflection coefficient of a laser signal in patients with an active inflammatory process in the vertebrae, which makes it possible to establish a differential diagnosis of the above conditions and to determine the time course of changes in the specific process in the vertebral column during therapy. Comparative analysis of the proposed method with the thermographic technique used in clinical practice demonstrated that the validity of laser biophotometry was highly competitive with that of thermography.

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