RESUMEN
Immunologic examinations were performed in 111 patients with uveitis concurrent with a variety of systemic pathologies, including 85 males and 26 females, mean age--32.4 +/- 5.8. A higher IL-6 content in blood serum (BS) and in lachrymal fluid (LF) was detected in all patients. The IL-4 content was lower in uveitis associated with ankylosing spondylitis and with Reuter's disease, whereas, it was higher in juvenile rheumatoid arthritis. The systemic immunity changes were related with the hemostasis impairments in the phagocytic, humoral and cellular chains: an enhanced functional activity of phagocyte cells, a lower absolute quantity of T-lymphocytes, a higher count of B-cells, a reduced concentration of IgM and of circulating immune complexes (CIC), and a diminished general hemolytic activity of the complement in BS. A reduced concentration of the secretory fraction of IgA, IgG and IgM concurrent with higher concentrations of IgA, C3- and C4 complement components, CIC and of lysozyme activity in LF was detected in the local immunity system.
Asunto(s)
Uveítis/complicaciones , Uveítis/inmunología , Adulto , Complejo Antígeno-Anticuerpo/inmunología , Artritis Juvenil/complicaciones , Artritis Reactiva/complicaciones , Linfocitos B/inmunología , Interpretación Estadística de Datos , Femenino , Humanos , Inmunoglobulina A/análisis , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Interleucina-4/sangre , Interleucina-6/sangre , Recuento de Linfocitos , Masculino , Muramidasa/análisis , Fagocitos/inmunología , Espondilitis Anquilosante/complicaciones , Linfocitos T/inmunología , Lágrimas/inmunologíaRESUMEN
Local immunity was studied in 81 patients (146 eyes) aged 11-49 with keratoconus at different stages of the disease running different course. The concentrations of IgG, IgM, secretory IgA, lysozyme, immune complexes, C3 component of the complement, transferrin, and alpha 1-antitrypsin are increased in tears of patients with keratoconus, reflecting the activation of local immunity in this condition.
Asunto(s)
Queratocono/inmunología , Adolescente , Adulto , Complejo Antígeno-Anticuerpo , Niño , Complemento C3/análisis , Interpretación Estadística de Datos , Humanos , Inmunoglobulinas/análisis , Persona de Mediana Edad , Nefelometría y Turbidimetría , Lágrimas/inmunología , Transferrina/análisis , alfa 1-Antitripsina/análisisRESUMEN
Total-systems and local immunity parameters were assessed in the lacrimal fluid of 118 patients with nonproliferative and proliferative diabetic retinopathy (DR). Humoral and cellular immunity characteristics of the total-systems immunity were shifted and local immune homeostasis impaired. The most manifest changes were detected in patients with proliferative DR. The authors hypothesize a possible contribution of local immune disorders to the pathogenesis of DR.
Asunto(s)
Retinopatía Diabética/inmunología , Lágrimas/inmunología , Adolescente , Adulto , Anciano , Formación de Anticuerpos/inmunología , Linfocitos B/inmunología , Complemento C3/inmunología , Complemento C4/inmunología , Femenino , Humanos , Inmunidad Celular/inmunología , Inmunoglobulinas/inmunología , Masculino , Persona de Mediana Edad , Linfocitos T/inmunologíaRESUMEN
Antigenic preparations from human tumour (TMG) and normal mammary gland (NMG) tissues were isolated, using water extraction followed by precipitation of proteins with perchloric acid, which were further separated into fractions A, B and C by gel filtration on Sepharose 6B. Glycoprotein fractions B and C were further separated by IEC on DEAE cellulose to give fractions B1-BIV and C1-CIV. A set of glycoproteins (Mr = 13 000-122 000; pI approximately 3-7.5) were detected in TMC and NMG, the glycoproteins specific for TMG or NMG as well as common for both TMG and NMG being found. The major glycoproteins (Mr = 46 000), i. e. GP-1 from TMG not found in NMG and GP-2 and GP-3 from NMG not found in TMG were isolated and characterized both biochemically and serologically.