RESUMEN
A pet domestic ferret (Mustela putorius furo) with a papular lesion involving the right pinna was diagnosed with chronic pyogranulomatous dermatitis by histopathologic examination. Intralesional, intracytoplasmic oval microorganisms compatible with Leishmania spp. or Histoplasma spp. were observed in macrophages and multinucleate giant cells. Leishmania infantum (L. infantum) infection was diagnosed by PCR, culture in Novy-MacNeal-Nicolle medium, and immunohistochemistry. Abnormal clinicopathological results included increased alanine transferase, alkaline phosphatase, serum gamma glutamyl transferase and polyclonal gammpathy. Anti-Leishmania antibodies were detected by enzyme-linked immunosorbent assay, immunofluorescence antibody test and western blot using L. infantum antigen. Immunoreactivity against the 16 kDa specific L. infantum antigen fraction was observed by western blot. PCR performed in blood samples obtained from this patient after positive parasite isolation detected L. infantum DNA. To the authors' knowledge, this is the first diagnosis and isolation of L. infantum in a domestic ferret naturally infected in an endemic region (Spain) where canine and feline leishmaniosis is frequently detected. According to these findings, ferrets should be included as potential reservoir hosts of L. infantum. Future investigations should analyze the epidemiological role of ferrets in L. infantum infection including the prevalence of infection.
Asunto(s)
Hurones , Leishmania infantum/inmunología , Leishmaniasis Visceral/veterinaria , Animales , Antígenos de Protozoos/sangre , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/parasitología , Reacción en Cadena de la Polimerasa/veterinaria , EspañaRESUMEN
Leishmaniosis is present in the Mediterranean region of Europe, where Leishmania infantum is responsible for the disease, dogs are the main reservoir, and sand flies of the Phlebotomus genus, subgenus Larroussius, are proven vectors. Some areas, including Minorca in the Balearic Islands, are considered free of the disease, despite the presence of vectors. However, in the context of the current expansion of canine leishmaniosis in parts of Europe, an epidemiological study using a veterinary questionnaire was carried out to establish the current situation of the disease in the Balearic Islands. While 50% of veterinarians thought that the incidence of canine leishmaniosis had not changed over time, 26.2% perceived an increasing trend, mainly those from Minorca, where most of the veterinarians polled (88.1%) considered the new diagnosed cases as autochthonous. A cross-sectional serological study performed in this island gave a seroprevalence rate of 24%. Seroprevalence among animals of local origin and with no history of movements to endemic areas was 31%. The presence of autochthonous canine leishmaniosis in Minorca was not correlated with an increase in vector density. The environmental and climatic factors that influenced the distribution and density of Phlebotomus perniciosus on the island and the possible causes of the apparent emergence of canine leishmaniosis in Minorca are discussed.
Asunto(s)
Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/parasitología , Leishmania infantum/aislamiento & purificación , Leishmaniasis Visceral/veterinaria , Animales , Estudios Transversales , Recolección de Datos , Perros , Femenino , Incidencia , Insectos Vectores , Leishmaniasis Visceral/epidemiología , Leishmaniasis Visceral/parasitología , Masculino , Phlebotomus/crecimiento & desarrollo , Estudios Prospectivos , Estudios Seroepidemiológicos , España/epidemiología , Encuestas y CuestionariosRESUMEN
Canine leishmaniasis (CanL) is a widespread disease present in 42 countries. It is considered of epidemiological importance because of its role as a reservoir of human leishmaniasis. Knowledge of the real distribution of CanL and its emergence and/or re-emergence is of great importance in order to determine the extension of the disease. This work reports the detection of CanL in a farm dog located in a Pyrenean area of northwest Catalonia (Spain) where the disease was previously unknown. Since the dog had never left the region and sandfly vectors, Phlebotomus ariasi and P. perniciosus, were present in the farm the case is considered as autochthonous and is the first to be published in this region of Spain.
Asunto(s)
Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/parasitología , Leishmania infantum/aislamiento & purificación , Leishmaniasis/veterinaria , Animales , Anticuerpos Antiprotozoarios/sangre , Western Blotting , Reservorios de Enfermedades , Enfermedades de los Perros/transmisión , Perros , Ensayo de Inmunoadsorción Enzimática , Humanos , Leishmania infantum/inmunología , Leishmaniasis/epidemiología , Leishmaniasis/transmisión , Leishmaniasis Visceral/veterinaria , España/epidemiologíaRESUMEN
During July 2007 sand fly captures were carried out in Andorra using sticky castor oil traps set in sand fly resting places for four consecutive nights. The sampling stations were located between 800 and 2400 m above sea level. The specimens captured belong to two species of the genus Phlebotomus subgenus Larroussius, Phlebotomus ariasi and Phlebotomus perniciosus. The results shed new light on the wide geographical and altitudinal distribution of P. ariasi in Andorra, where it was located between 800 and 2200 m a.s.l. The study also identified P. perniciosus in Andorra for the first time, with captures below 1000 m a.s.l. The finding of these species, both proven vectors of human and canine leishmaniasis in the bordering areas of France and Spain, is considered in terms of a possible emergence of leishmaniasis in Andorra, as has occurred in other parts of Europe.