Asunto(s)

Ampirona/análisis , Antipirina/análogos & derivados , Compuestos de Anilina , Fenómenos Químicos , Química , Indicadores y Reactivos , Soluciones/análisis , Espectrofotometría Ultravioleta

RESUMEN

Computer-assisted optimization of a high-performance liquid chromatograph and associated post-column reactor is reported for the determination of six amino-acids. First six, then seven, experimental variables were considered. Non-standard experimental conditions were found which gave significantly improved colour development in the ninhydrin reaction. The composite modified simplex method for experimental optimization and a novel system-response function facilitated rapid and simultaneous improvement of separation, sensitivity and analysis time. The approach described is directly applicable to many similar systems.