RESUMEN

Turcinoemacheilus ekmekciae, new species, from upper Euphrates and Tigris drainages is distinguished from other species of Turcinoemacheilus in Western Asia by having a dark stripe broader than the eye diameter along the lateral line, rarely possessing roundish blotches, 5-6 mandibular pores in mandibular canal, a comperatvely smaller head, a deeper body, and a greater pre-pelvic distance. Our specimens collected from the upper Great Zab, near the type locality of Turcinoemacheilus kosswigi, showed notable genetic divergence (a minimum K2P of 3.3%) from sequences reported as T. kosswigi in previous studies. Despite morphological similarities, this molecular difference suggests that the populations analysed in previous studies may represent a potential new species of Turcinoemacheilus, which we tentatively named as Turcinoemacheius cf. kosswigi. Molecular data also suggest that T. ekmekciae is characterized by a minimum K2P distance of 3.5% from Turcinoemacheilus minimus and T. cf. kosswigi. The three methods for species delimitation (assemble species by automatic partitioning [ASAP], Poisson tree processes [PTP], and multi-rate PTP [mPTP]) that were utilized for testing species assignments consistently identified our test group as a distinct species.

Asunto(s)

Cipriniformes , Ríos , Animales , Flujo Genético

RESUMEN

Phoxinus abanticus, a new species, is described from the Lake Abant basin. It is distinguished from Phoxinus species in Türkiye and adjacent waters by the presence of fewer lateral line scales (60-69, vs. 75-91 in Phoxinus colchicus, 75-90 in Phoxinus strandjae); a deeper caudal peduncle (caudal peduncle depth: 1.8-2.3 times in length, vs. 2.4-2.9 in P. colchicus; 2.5-3.2 in P. strandjae); the absence of scales in the breast of males (vs. present); and ventral body reddish in nuptial colouration pattern for male (vs. brackish). The new species, P. abanticus, is also distinguished from its closest relative, P. strandjae, by a minimum of 3.40% genetic distance in the mtDNA cytochrome b (cyt b) gene.

Asunto(s)

Cyprinidae , Cipriniformes , Masculino , Animales , Lagos , Ríos , Cipriniformes/genética , ADN Mitocondrial/genética , Citocromos b , Cyprinidae/genética

RESUMEN

The DNA barcoding approach was used for the determination of evolutionary relationships and species delimitation of the genus Oxynoemacheilus (Teleostei: Nemacheilidae). The COI barcode region (615 bp amplicon) was used to barcode 444 individuals from 64 morphologically identified species in the genus Oxynoemacheilus and 189 haplotypes were identified. The average of the interspecific p distance (9.59%) was about 21-fold higher than the average intraspecific distance (0.44%). A general genetic threshold of 1.46% sequence divergence was defined for species delimitation. The multiple species delimitation methods (BCM, GMYC, bPTP and TCS) revealed a total of 62 molecular operational taxonomic units for 64 morphospecies with a new loach species from the BuyukMelen River. Neighbour-joining, maximum likelihood and Bayesian inference analyses indicated that all haplotypes were clustered into 62 clades, which corresponded to Oxynoemacheilus species, with strong bootstrap support (≥95%). Furthermore, all samples grouped in concurrence with the taxonomic status of the species except for species groups (O. germencicus-O. cinicus-O. mesudae and O. leontinae-O. namiri) that were showed intraspecific overlap in genetic diversity for COI-based barcodes. In conclusion, our analyses indicate that COI-based barcodes provide reliable species discrimination. Therefore, we currently recommend COI barcodes as the suitable barcode for genus Oxynoemacheilus.

Asunto(s)

Cipriniformes , Código de Barras del ADN Taxonómico , Animales , Teorema de Bayes , ADN , Código de Barras del ADN Taxonómico/métodos , Filogenia , Turquía

RESUMEN

Turcichondrostoma, a new genus, from the Southwestern Anatolia is distinguished by having fewer gill rakers on first gill arch and morphologies of premaxilla and dentary bones. In addition, as a result of the phylogenetic analyses based on combine data set (mtDNA COI + Cytb) sequences (1706 bp.), Turcichondrostoma genus was recovered with high posterior probability value (BI PP:1.0) and strong-supported bootstrap value (ML BP: 100%) among the former Chondrostoma groups. Also, high K2P mean genetic distance values (more than 7.84%) differentiated genus Turcichondrostoma from the other genera of former Chondrostoma group. The results of both morphological-osteological and molecular analyses are congruent with each other. The results of this study revealed that the genus Turcichondrostoma is easily distinguished from the genera in Chondrostoma group.

Asunto(s)

Cipriniformes , Animales , Cipriniformes/genética , ADN Mitocondrial , Branquias , Filogenia , Turquía

RESUMEN

In this study, the phylogeny of Alburnus genus distributed in Turkish freshwaters was performed by analyzing mitochondrial cyt b gene (1141 bp) and COI gene (1551 bp) sequences from 1172 samples representing 112 populations of 24 species through their geographical distribution. According to our findings, 20 valid species are distributed in Turkey of which 18 have already been known. While six Alburnus species (A. battalgilae, A. istanbulensis, A. carinatus, A. schischkovi, A. nasreddini ve A. adanensis) have been synonomized, two new species (Alburnus sp.1 and Alburnus sp.2) from Dicle River and Çapraz Stream/Susurluk River have been identified. Extinct species such as A. akili and A. nicaeensis have not been observed in situ. Phylogenetic tree topologies and haplotype network of the 119 cyt b and 80 COI haplotypes detected in Alburnus species have indicated a consensus tree topology containing twenty lineages, each of corresponding to one species, and three Alburnus haplogroups corresponding to the geographical origins: Eastern Anatolia (I), Mediterranean (2) and Western & Northern Anatolia (3). The results indicate that the divergence between those haplogroups may have occurred during the Middle Miocene-Middle Pleistocene periods (from 14.9 to 5.29 million years).

Asunto(s)

Cyprinidae/clasificación , Citocromos b/genética , Complejo IV de Transporte de Electrones/genética , Análisis de Secuencia de ADN/métodos , Animales , Cyprinidae/genética , Haplotipos , Mar Mediterráneo , Filogenia , Filogeografía , Turquía

RESUMEN

Turkey has a rich freshwater biodiversity in terms of Cyprinid genus in respect to its geographical location. To elucidate the phylogeny of the Alburnoides genus, one of these genera, genetic data for the cytochrome b gene (1141 bp) was generated for 445 samples collected at 42 sampling sites across their geographical distribution. A total of 54 mitochondrial haplotypes identified were distrubuted among distinct twelve species that did not share haplotypes with each other. Pairwise sequence divergence among these species range from 1.37% (A. emineae and A. velioglui) and 10.99% (A. manyasensis and A. smyrnae). A new potential species in the River Dirgine that run into the Black Sea Basin was separated from the most closed known species with mean 6.3%. Network analysis and phylogenetic analysis indicated that all haplotypes were clustered into two major clades, which corresponded to twenty-three Alburnoides lineages, with moderate-high bootstrap supports and mutational steps, respectively. Application of a molecular clock to a Bayesian phylogeny indicates that Alburnoides diversified under the paleogeographic conditions such as tectonic uplift and faulting Miocene aged as well as climatic oscillation and sea-level fluctuations during late Miocene-middle Pleistocene. The genetic results of the present study indicated the inter-specific distance of cyt b gene sequences followed the ideal results for species identification and phylogeny of Turkish spirlins.

Asunto(s)

Cyprinidae/genética , ADN Mitocondrial/genética , Genoma Mitocondrial/genética , Análisis de Secuencia de ADN , Animales , Filogenia , Filogeografía , Especificidad de la Especie , Turquía

RESUMEN

Oxynoemacheilus cemali sp. nov. is described from the Çoruh River drainage in the eastern Black Sea basin. One molecular marker (coI), 25 morphometric and four meristic characters were analysed. Oxynoemacheilus cemali is distinguished from O. kosswigi, O. banarescui, O. samanticus and O. angorae in the Black Sea basin by having a suborbital groove in males, an axillary lobe at the pelvic-fin base, no dorsal adipose crest on the caudal peduncle, a slightly-forked caudal fin and 7-15 dark grey dorsal saddles. Morever, Oxynoemacheilus cemali is distinguished by commonly having 9-15 irregularly-shaped dark-grey bars on the flank posterior to the dorsal-fin origin or, rarely having a mottled pattern or 4-6 irregularly shaped dark-grey bars on the flank posterior to the dorsal-fin origin. Oxynoemacheilus cemali is also distinguished from the closely related species O. araxensis and O. cyri, distributed outside the Black Sea basin, by having 15 and 31 diagnostic nucleotide substitutions in the coI barcode region, respectively.

Asunto(s)

Cipriniformes/clasificación , Distribución Animal , Animales , Color , Cipriniformes/anatomía & histología , Cipriniformes/genética , Femenino , Masculino , Filogenia , Pigmentación , Ríos , Turquía

RESUMEN

Gobio baliki, new species, is described from the stream Büyük Melen in the northwestern Anatolian Black Sea basin. The new species is distinguished from other gudgeons in Anatolia by the breast being completely covered by scales, scales extending slightly behind the isthmus; 5-6 scales between the posterior extremity of the pelvic-fin base and the anus; the distance between the anus and the anal-fin origin 7-10% SL; the postorbital distance equal to or greater than the snout length; the depth of the anal-fin approximately equal to the length of the pelvic-fin; 8-9 midlateral black blotches, usually separated from each-other.

Asunto(s)

Cyprinidae , Animales , Mar Negro , Ríos , Turquía

RESUMEN

PURPOSE: The Fujimori gate flap is an innervated flap raised from the nasolabial area for reconstruction of the lower lip. No electromyographic or clinical long-term studies have analyzed the long-term outcomes of lower lip reconstruction performed using this method. The aim of the present study was to assess the outcomes of lower lip reconstruction with the Fujimori gate flap using clinical and electrophysiologic examinations. PATIENTS AND METHODS: Ten patients who had been treated with this procedure were evaluated clinically and electrophysiologically. The follow-up period was 1 year for all patients, and the patients underwent regular assessments. RESULTS: All flaps survived completely, and no wound healing problems were encountered. Four patients underwent revision in the late postoperative period. The electrophysiologic studies revealed the presence of reinnervation in all 10 patients. CONCLUSIONS: In the present study, we found that the Fujimori gate flap is a versatile flap for ideal reconstruction of lower lip defects. Our electrophysiologic assessments showed that the transferred muscle had undergone reinnervation and that the donor area innervation had been preserved. The findings from the serial clinical and electrophysiologic assessments indicated satisfactory results.

Asunto(s)

Labio/cirugía , Procedimientos de Cirugía Plástica/métodos , Colgajos Quirúrgicos/trasplante , Potenciales de Acción/fisiología , Adulto , Anciano , Carcinoma de Células Escamosas/cirugía , Ingestión de Alimentos/fisiología , Electromiografía/métodos , Estética , Femenino , Estudios de Seguimiento , Supervivencia de Injerto , Humanos , Labio/inervación , Labio/fisiología , Neoplasias de los Labios/cirugía , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Neuronas Motoras/fisiología , Regeneración Nerviosa/fisiología , Conducción Nerviosa/fisiología , Satisfacción del Paciente , Reoperación , Estudios Retrospectivos , Sensación/fisiología , Habla/fisiología , Resultado del Tratamiento

Asunto(s)

Traumatismos de los Dedos/cirugía , Traumatismos de los Tendones/cirugía , Tendones/cirugía , Dimetilpolisiloxanos , Humanos , Prótesis e Implantes

RESUMEN

BACKGROUND: Rhinoplasty can be performed using both open and closed approaches. A visible scar on the columella is the major disadvantage of open rhinoplasty. Different columellar incision types have been used in open rhinoplasty. In this retrospective study, we compare transverse and inverted-V columellar incisions with a scar assessment scale. METHODS: In this retrospective study, open rhinoplasty was performed on 84 patients between 2001 and 2006. The transverse incision was used on 39 patients (18 males, 21 females). The inverted-V incision was used on 45 patients (21 males, 24 females). The entire surgical procedure was performed by a single surgeon. The columellar incision was closed using 6-0 interrupted polypropylene sutures. All sutures were removed on the fifth postoperative day. With this assessment scale, we observed satisfactory scar, pigmentation, and notching. RESULTS: We compared both groups and found that the inverted-V incision resulted in better scar formation (p < 0.05) and less notching (p < 0.07). Scar pigmentation was found to be irrelevant to the incision technique employed (p < 0.3). CONCLUSION: As a result of this study we concluded that the inverted-V incision might be a better choice in open rhinoplasty.

Asunto(s)

Cicatriz/patología , Rinoplastia/métodos , Adulto , Cicatriz/etiología , Femenino , Humanos , Masculino , Nariz