RESUMEN
A highly simple, sensitive, specific and low-cost electrochemical apta-cyto-sensor for determination of circulating human MDA-MB-231 breast cancer cells was fabricated. Non-spherical nanoparticles of gold were electro-deposited in the presence of ethosuximide as a shape directing and size controlling agent. The nanoparticles had dimensions ranging 50-150â¯nm, and covered the underlying surface with a roughness factor of 8.03. The Non-spherical nanoparticles were then employed as the apta-cyto-sensor transducer. A 83-mer DNA aptamer that is specific to capturing the cell surface proteins was immobilized on the transducer surface, and binding with the cells was followed using the ferro/ferricyanide redox marker. The aptamer was immobilized within â¼200â¯min on the transducer surface. The cells were quantified with an equation of regression of ΔIp(µA) = (1.028⯱â¯0.027) log (C (cell mL-1)) + (0.2199⯱â¯0.0944), a sensitivity of 1.028⯵A (log (concentration / cell mL-1))-1 and a quantitation limit of 2 cell mL-1, in a concentration range of 5 to 2â¯×â¯106 cell mL-1. The apta-cyto-sensor selectivity was also evaluated toward AsPC-1, Calu-6, HeLa, MCF-7 and melanoma B16/F10 cell lines. The apta-cyto-sensor had a fabrication reproducibility of 4.2%, regeneration capability of 5.1%, a stability of 35 days, and a potential application for the detection of MDA-MB-231 cells in the spiked blood serum samples with a sensitivity of 0.8975⯵A (log (concentration / cell mL-1))-1 and a quantitation limit of 5 cell mL-1, in a concentration range of 10 to 1â¯×â¯103 cell mL-1. The apta-cyto-sensor would be applicable for breast cancer diagnosis at early stage.
Asunto(s)
Técnicas Biosensibles/métodos , Neoplasias de la Mama/tratamiento farmacológico , Técnicas Electroquímicas/métodos , Oro/administración & dosificación , Nanopartículas del Metal/administración & dosificación , Aptámeros de Nucleótidos/administración & dosificación , Línea Celular Tumoral , ADN/administración & dosificación , Electrodos , Femenino , Células HeLa , Humanos , Límite de Detección , Células MCF-7 , Melanoma Experimental , Reproducibilidad de los ResultadosRESUMEN
A self-nanoemulsifying drug delivery system (SNEDDS) was developed as a novel route to enhance the efficacy of docetaxel lipophilic drug. SNEDDS comprised ethyl oleate, Tween 80 and poly(ethylene glycol) 600, as oil, surfactant and co-surfactant, and formed stabilized monodispersed oil nanodroplets upon dilution in water. SNEDDS represented encapsulation efficiency and loading capacity of 21.4 and 52.7%, respectively. The docetaxel release profile from the drug-loaded SNEDDS was recorded, its effectiveness against MCF-7 cell line was investigated, and an IC50 value of 0.98 ± 0.05 µg mL-1 was attained. The drug-loaded SNEDDS was administrated in rats, and the pharmacokinetic parameters of maximum concentration of 22.2 ± 0.8 µg mL-1, time to attain this maximum concentration of 230 min, and area under the curve of 1.71 ± 0.18 µg min mL-1 were obtained. The developed SNEDDS formulation can be represented as an alternative to docetaxel administration.