RESUMEN
This study aimed to compare the effectiveness of the ovarian lavage / artificial insemination method with the traditional hormonal administration and fertilization methods over the artificial reproduction of the northern pike (Esox lucius). For this purpose, groups of five females were treated as follows: intraperitoneal injection of saline (C1); ovarian lavage with saline (C2); intraperitoneal injection of carp pituitary extract (CPE, T1); ovarian lavage with CPE (T2); intraperitoneal injection of CPE and ovarian lavage with semen after 72â¯h (T3); ovarian lavage with CPE and ovarian lavage with semen after 72â¯h (T4). According to the results, no fish ovulated in the control groups (C1 and C2). There were no significant differences (n.s.) among experimental treatments (P > 0.05, n.s.) in the reproductive parameters, such as latency time, ovulation rate, stripped egg amount, and pseudo-gonadosomatic index. The lowest fertilization rate (54.8%) was observed in the T4 treatment and significantly differed from the T1 and T2 treatments (P < 0.05). Moreover, the highest survival at swim-up stage was measured in the T4 treatment with a significant difference compared to the T1 group (P < 0.05). The survival at the eyed-egg stage, hatching rate, and malformations were similar (P > 0.05, n.s.) in all applied strategies. The results demonstrated that the ovarian lavage / artificial insemination method could be applied to control northern pike reproduction, like the traditional fertilization method. Consequently, this novel technique can be suggested as an alternative strategy to facilitate the hatchery operations in the controlled reproduction of this species.
Asunto(s)
Esocidae , Irrigación Terapéutica , Femenino , Animales , Irrigación Terapéutica/veterinaria , Reproducción , Inseminación Artificial/veterinaria , PecesRESUMEN
The present study aimed to determine the effects of dietary probiotic supplementation on feed efficiency, physiological parameters, and semen quality of male rainbow trout (Oncorhynchus mykiss) broodstock. For this purpose, a total of 48 breeders with an average initial weight of 1366.1 ± 33.8 g were divided into 4 groups and 3 replicates. Fish were fed with diets containing 0 (control), 1 × 109 (P1), 2 × 109 (P2), and 4 × 109 (P3) CFU multi-strain probiotic kg-1 diet for 8 weeks. According to the results, P2 treatment significantly enhanced body weight increase, specific growth rate, and protein efficiency ratio and decreased feed conversion ratio. Moreover, the highest values of red blood cells count, hemoglobin, and hematocrit values were observed in P2 treatment (P < 0.05). The lowest levels of glucose, cholesterol, and triglyceride were found in P1, P2, and P3 treatments, respectively. Also, the highest levels of total protein and albumin were obtained in P2 and P1 treatments (P < 0.05). Based on the results, plasma enzymes contents were significantly decreased in P2 and P3 treatments. In terms of immune parameters, the complement component 3, complement component 4, and immunoglobulin M levels were increased in all probiotic-fed treatments (P < 0.05). For spermatological features, the highest spermatocrit value, sperm concentration, and motility time were observed in the P2 treatment (P < 0.05). Consequently, we conclude that multi-strain probiotics can be used as functional feed additives in male rainbow trout broodstock to enhance semen quality, improve physiological responses, and better feed efficiency.
Asunto(s)
Oncorhynchus mykiss , Probióticos , Masculino , Animales , Análisis de Semen/veterinaria , Oncorhynchus mykiss/fisiología , Semen/química , Probióticos/farmacología , Dieta/veterinaria , Inmunidad Innata , Alimentación Animal/análisis , Suplementos DietéticosRESUMEN
The present experiment aims to study the effects of dietary multi-strain probiotics on semen quality, seminal plasma compositions, and fertilization ability of male rainbow trout. For this purpose, a total of 48 broodstocks with an average initial weight of 1366.1 ± 33.8 g were divided into 4 groups and 3 replicates. Fish were fed with diets containing 0 (control), 1 × 109 (P1), 2 × 109 (P2), and 4 × 109 (P3) CFU probiotic kg-1 diet for 12 weeks. Results showed that dietary supplementation of probiotics significantly increased plasma testosterone level, motility time of spermatozoa, sperm density, and spermatocrit value in P2 and P3 treatments and Na+ level in P2 treatment compared to the control group (P < 0.05). The activities of aspartate aminotransferase and lactate dehydrogenase had significantly decreased in the P2 treatment compared to the control group (P < 0.05). No considerable variations were observed between control fish and treatment groups (P > 0.05) in semen biochemical parameters, percentage of motile spermatozoa, osmolality, and pH of seminal plasma. Based on the results, the highest fertilization rate (97.2 ± 0.9%) and eyed egg survival (95.7 ± 1.6%) were observed in the P2 treatment, and those values showed remarkable differences with the control group (P < 0.05). The results indicated that multi-strain probiotics have potential efficacy on semen quality and fertilization ability of rainbow trout broodstock spermatozoa.