RESUMEN
Spontaneously hypertensive rats (SHR) are widely used as a model of attention deficit hyperactivity disorder (ADHD) as their ADHD-like behaviors are restored by methylphenidate. However, a postnatal neural development in SHR is unknown. We performed whole cell patch clamp recordings from locus coeruleus (LC) neurons in neonatal [postnatal day (P) 3-5], juvenile (P21-28), and adult (P 49-56) SHR and age-matched Wistar rats to evaluate α1- and α2-adrenergic receptor (ARs) activities at each developmental period. LC neurons in neonatal Wistar rats and SHR showed no difference in resting membrane potential and spontaneous firing rate, while juvenile and adult SHR LC neurons showed depolarized resting membrane potential and faster spontaneous firing rate than in Wistar rats. Blockade of α1-AR activity by prazosin hyperpolarized the membrane and abolished spontaneous firings in all developmental periods in SHR LC neurons, but not in juvenile and adult Wistar rats. α1-AR stimulation by phenylephrine evoked an inward current in juvenile LC neurons in SHR, but not in juvenile Wistar rats. This phenylephrine-induced inward current was abolished by nonselective cation channel blockers. By contrast, α2-AR stimulation-induced outward currents in the presence of an α1-AR antagonist were equivalent in SHR and Wistar LC neurons. These data suggest that Wistar LC neurons lose α1-AR function during development, whereas α1-ARs remain functional in SHR LC neurons. Thus persistent intrinsic activity of α1-ARs may be a neural mechanism contributing to developmental disorders in juvenile SHRs.
Asunto(s)
Potenciales de Acción , Trastorno por Déficit de Atención con Hiperactividad/fisiopatología , Locus Coeruleus/fisiología , Receptores Adrenérgicos alfa 1/metabolismo , Agonistas de Receptores Adrenérgicos alfa 1/farmacología , Antagonistas de Receptores Adrenérgicos alfa 1/farmacología , Factores de Edad , Animales , Trastorno por Déficit de Atención con Hiperactividad/metabolismo , Locus Coeruleus/citología , Locus Coeruleus/crecimiento & desarrollo , Locus Coeruleus/metabolismo , Potenciales de la Membrana , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Neuronas/fisiología , Fenilefrina/farmacología , Prazosina/farmacología , Ratas , Ratas Endogámicas SHR , Ratas WistarRESUMEN
Effects of edaravone, a free radical scavenger, on post-traumatic impairment of long-term potentiation (LTP) were examined in granule cell layers of the dentate gyrus (DG) in vitro. Field EPSPs (fEPSPs) evoked by stimulation of the perforant path (PP) were recorded extracellularly in the DG one week after a moderate impact applied by a fluid percussion injury (FPI) device. High frequency stimulation (HFS) of the PP caused LTP of the fEPSP-slope in slices from naïve and sham-operated rats, however, the LTP was strongly depressed in slices from FPI rats. Intraperitoneal administration of edaravone 15 min after FPI prevented the hyperactivities of DG neurons and attenuated impairment of the LTP in FPI rat dentate granular cells. In vitro application of spermine NONOate (sp-NO), a nitric oxide (NO) donor, for 30 min produced a gradual increase in the fEPSP-slope, lasting for more than 2 h. Edaravone attenuated the enhancement of the fEPSP-slope induced by sp-NO. After sp-NO treatment HFS could not produce an obvious LTP in the DG granule cell layer. Pretreatment of DG slices with edaravone prevented the sp-NO-induced impairment of LTP. These results suggest that administration of edaravone after FPI protects against post-traumatic impairment of LTP in granule cell layers of the DG, possibly by scavenging NO-related radicals.
Asunto(s)
Antipirina/análogos & derivados , Lesiones Encefálicas/fisiopatología , Giro Dentado/efectos de los fármacos , Depuradores de Radicales Libres/farmacología , Plasticidad Neuronal/efectos de los fármacos , Sinapsis/efectos de los fármacos , Animales , Antipirina/administración & dosificación , Antipirina/farmacología , Lesiones Encefálicas/patología , Giro Dentado/patología , Giro Dentado/fisiología , Edaravona , Estimulación Eléctrica , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Depuradores de Radicales Libres/administración & dosificación , Inyecciones Intraperitoneales , Potenciación a Largo Plazo/efectos de los fármacos , Potenciación a Largo Plazo/fisiología , Masculino , Modelos Animales , Plasticidad Neuronal/fisiología , Donantes de Óxido Nítrico/farmacología , Ratas , Ratas Wistar , Espermina/análogos & derivados , Espermina/farmacología , Sinapsis/fisiologíaRESUMEN
The present study examined the effect of methylphenidate (MPH), a psychostimulant, on nor-adrenergic transmission in the locus coeruleus (LC) of juvenile rats. Intracellular recordings showed that MPH (>3 µM) produced a hyperpolarizing response associated with a decrease in the rate of spontaneously firing action potentials. MPH (1 µM) enhanced the amplitude of the inhibitory postsynaptic potential (IPSP) mediated by norepinephrine (NE), but did not change the excitatory postsynaptic potential (EPSP) mediated by excitatory amino acids. Whole-cell patch-clamp recordings showed that MPH (0.3-30 µM) produced an outward current (I(MPH)) and enhanced the inhibitory postsynaptic current (IPSC) in neurons of the juvenile rat LC. MPH (30 µM) enhanced the NE-induced outward current (I(NE)). Bath-application of yohimbine (1 µM) produced an inward current and blocked the MPH-induced enhancement of the IPSC. Yohimbine (1 µM) depressed not only the I(NE) but also the I(MPH) in juvenile rat LC neurons. The current-voltage relationship of the I(MPH) showed inward rectification and reversed polarity at -91.1±4.3 mV (n=5). Ba(2+) (100 µM) blocked the I(MPH), indicating that the I(MPH) is mediated by Ba(2+)-sensitive inward rectifier K(+) current. These results suggest that MPH enhances inhibitory synaptic transmission by increasing the concentration of NE at noradrenergic synapses in juvenile rat LC neurons.
Asunto(s)
Estimulantes del Sistema Nervioso Central/farmacología , Locus Coeruleus/metabolismo , Metilfenidato/farmacología , Norepinefrina/metabolismo , Transmisión Sináptica/efectos de los fármacos , Animales , Potenciales Postsinápticos Excitadores , Técnicas In Vitro , Locus Coeruleus/fisiología , Masculino , Técnicas de Placa-Clamp , Ratas , Ratas Endogámicas WKYRESUMEN
The neurochemical phenotypes of the transient receptor potential melastatin-8 (TRPM8)-immunoreactive afferent neurons innervating the rat urinary bladder were examined by using a highly sensitive tyramide signal amplification method, combined with wheat-germ agglutinin-horseradish peroxidase (WGA-HRP) retrograde tracing. TRPM8-immunoreactivity was detected in a small proportion of the WGA-HRP-labeled bladder afferent neurons in the dorsal root ganglia of the Th13-L1 (1.14%) and the L6-S1 (1.27%), and these neurons were small in size (<600 microm(2)). The 82.6+/-3.8% of the TRPM8-immunoreactive bladder afferent neurons and 80.9+/-1.5% of the total population of the TRPM8-immunoreactive afferent neurons in the observed dorsal root ganglia expressed NF200. On the other hand, the proportions of the co-expression of TRPM8 and nociceptive markers such as calcitonin gene-related peptide (CGRP), transient receptor potential vanilloid-1 (TRPV1), and isolectin B4 (IB4) in the bladder afferent neurons (81.5+/-5.2% for CGRP, 36.1+/-4.0% for TRPV1, and 15.8+/-5.5% for IB4) were higher in comparison to those in the total population of the TRPM8-immunoreactive afferent neurons (21.9+/-2.4% for CGRP, 16.6+/-1.7% for TRPV1, and 5.4+/-0.5% for IB4), although no significant difference existed for IB4. Our results suggest that the TRPM8-expressing bladder afferents should be classified as Adelta-fibers and C-fibers, while some of these afferents may be involved in nociceptive sensations.
Asunto(s)
Ganglios Espinales/metabolismo , Nociceptores/metabolismo , Células Receptoras Sensoriales/metabolismo , Canales Catiónicos TRPM/metabolismo , Vejiga Urinaria/inervación , Aferentes Viscerales/metabolismo , Animales , Biomarcadores/metabolismo , Péptido Relacionado con Gen de Calcitonina/metabolismo , Recuento de Células , Femenino , Ganglios Espinales/citología , Inmunohistoquímica , Fibras Nerviosas Mielínicas/metabolismo , Fibras Nerviosas Mielínicas/ultraestructura , Fibras Nerviosas Amielínicas/metabolismo , Fibras Nerviosas Amielínicas/ultraestructura , Técnicas de Trazados de Vías Neuroanatómicas/métodos , Proteínas de Neurofilamentos/metabolismo , Nociceptores/citología , Dolor/metabolismo , Dolor/fisiopatología , Lectinas de Plantas , Ratas , Ratas Wistar , Células Receptoras Sensoriales/citología , Canales Catiónicos TRPV/metabolismo , Aferentes Viscerales/citología , Aglutinina del Germen de Trigo-Peroxidasa de Rábano Silvestre ConjugadaRESUMEN
The present experiment examined the effect of mild hypothermia (35 degrees C) on the post-traumatic hyperactivity of rat hippocampal CA1 neurons in horizontal brain slices. One week after fluid percussion injury (FPI), the optical response evoked by stimulation of the Schaffer collaterals increased in amplitude and propagation area in hippocampal CA1 slices. FPI did not alter the fast optical response that reflected the action potential of the Schaffer collaterals but enhanced the slow component that reflected the excitatory postsynaptic response. FPI increased the slope of the input-output relation (I/O function), suggesting that FPI increases the efficacy of excitatory synaptic transmission in the hippocampal CA1 pyramidal neurons. To examine the effect of low temperature on post-traumatic hyperactivity of hippocampal CA1 neurons, mild hypothermia (35 degrees C) was administered to rats 15 min after FPI and maintained for 1-3 h. One week after FPI, the activity of hippocampal CA1 neurons in rats with mild hypothermia appeared to be reduced as compared with those receiving FPI alone. The post-traumatic enhancement of the I/O function of the slow optical response was prevented by mild hypothermia. These results suggest that mild hypothermia applied 15 min after FPI attenuates the post-traumatic hyperactivity of excitatory synapses in rat hippocampal CA1 neurons.
Asunto(s)
Lesiones Encefálicas/fisiopatología , Región CA1 Hipocampal/fisiopatología , Hipotermia Inducida , Transmisión Sináptica , Animales , Calcio/metabolismo , Potenciales Postsinápticos Excitadores , Masculino , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismoRESUMEN
The lateral septal nucleus receives a diffuse dopaminergic input originating from the ventral tegmental area of the brain stem. We examined whether dopamine (DA) modulates synaptic transmission in the slice preparation of the rat dorsolateral septal nucleus (DLSN). Bath application (10-15 min) of DA (30 muM) markedly depressed the amplitude of fast and slow inhibitory postsynaptic potentials (IPSPs) in DLSN neurons, while it produced only a minor depression of the amplitude of excitatory postsynaptic potentials (EPSPs) obtained in the presence of bicuculline. DA (30 muM) depressed the monosynaptic fast IPSP to approximately 50% of control, but did not depress the inward current (I(GABA)) induced by exogenous gamma-aminobutyric acid (GABA). DA decreased the frequency of miniature fast IPSPs (m-fIPSPs) without significantly changing their amplitude. PD 168077, a selective D4 receptor agonist, depressed the fast and slow IPSPs but not the EPSP and decreased the frequency of m-fIPSPs. Both DA and PD 168077 increased the paired-pulse ratio of the monosynaptic fast IPSP. The inhibitory effect of DA on the fast IPSP was significantly attenuated by L-741,742, an antagonist at D4 receptors, but not by SCH 23390 and sulpiride, a D1-like and a D2-like receptor antagonist, respectively. N-ethylmaleimide, a blocker of pertussis toxin (PTX)-sensitive G protein (G(i/o)), attenuated the DA-induced depression of the fast IPSP. N-[2-((p-bromocinnamyl) amino)ethyl]-5-isoquinoline sulfonamide, a protein kinase A (PKA) inhibitor, attenuated the DA-induced depression of the fast IPSP. These results suggest that DA inhibits spontaneous and evoked release of GABA via the D4 receptor-G(i)-protein-PKA system in DLSN neurons.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/fisiología , Dopamina/fisiología , Receptores de Dopamina D4/fisiología , Receptores Presinapticos/fisiología , Tabique del Cerebro/fisiología , Transducción de Señal/fisiología , Transmisión Sináptica/fisiología , Animales , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Dopamina/farmacología , Agonistas de Dopamina/farmacología , Electrofisiología , Inhibidores Enzimáticos/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Isoquinolinas/farmacología , Masculino , Ratas , Ratas Wistar , Receptores de Dopamina D4/efectos de los fármacos , Receptores Presinapticos/efectos de los fármacos , Tabique del Cerebro/citología , Tabique del Cerebro/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Sulfonamidas/farmacología , Transmisión Sináptica/efectos de los fármacosRESUMEN
We investigated the effects of traumatic brain injury (TBI) on the glutamatergic synaptic transmission in the hippocampal CA1 area. A moderate impact (3.8-4.8atm) was applied onto the left parietal cerebral cortex by a fluid percussion injury (FPI) device. Conventional intracellular recordings were made from hippocampal CA1 pyramidal neurons in vitro. Electrophysiological properties of these neurons were compared between three groups (control, FPI-ipsilateral, and FPI-contralateral). The excitability of postsynaptic membrane of CA1 pyramidal neurons was not altered by the moderate FPI; however, the evoked glutamatergic excitatory synaptic transmission in the pyramidal neurons of post-FPI-CA1 was enhanced. Paired-pulse facilitation (PPF) was significantly suppressed in both the FPI-ipsilateral and FPI-contralateral groups and the frequencies of mEPSPs in neurons from the bilateral FPI groups were greater than the frequency in the control group. These results suggest that the glutamatergic synaptic transmission in the hipppocampal CA1 area is facilitated through presynaptic mechanisms after TBI.
Asunto(s)
Lesiones Encefálicas/metabolismo , Epilepsia/metabolismo , Ácido Glutámico/metabolismo , Hipocampo/metabolismo , Terminales Presinápticos/metabolismo , Transmisión Sináptica/fisiología , Potenciales de Acción/fisiología , Animales , Lesiones Encefálicas/complicaciones , Lesiones Encefálicas/fisiopatología , Modelos Animales de Enfermedad , Epilepsia/etiología , Epilepsia/fisiopatología , Potenciales Postsinápticos Excitadores/fisiología , Hipocampo/fisiopatología , Masculino , Plasticidad Neuronal/fisiología , Células Piramidales/metabolismo , Ratas , Ratas WistarAsunto(s)
Dopamina/farmacología , Núcleos Septales/efectos de los fármacos , Animales , Masculino , Potenciales de la Membrana/efectos de los fármacos , Canales de Potasio de Rectificación Interna/efectos de los fármacos , Canales de Potasio de Rectificación Interna/fisiología , Ratas , Ratas Wistar , Núcleos Septales/fisiología , Transmisión Sináptica/efectos de los fármacosRESUMEN
BACKGROUND: 5-dihydroxyanthraquinone-2-carboxylic acid (rhein) inhibits oxidoreduction induced by reducing nicotingamide adenine dinucleotide in the mitochondria and reducing reactive oxygen species, it also suppresses lipid peroxidation in rat brain homogenates. This study was to assess the effects of anthraquinone derivatives, rhein on synaptic transmission in the rat hippocampal CA1 pyramidal cell layer by intracellular recording. METHODS: The excitatory postsynaptic potential (EPSP) evoked by stimulation of the Schaffer collaterals in the presence of bicuculline (15 micromol/L) was depressed by application of rhein (0.3 - 30 micromol/L). The amplitude of the EPSP was restored within 20 minutes after removal of rhein from the supernatant. At a concentration of 30 micromol/L, rhein reduced the amplitude of the EPSP to 42% +/- 3.7% (n = 24) of the control. Subsequently, wavelet spectral entropy was used to analyze the EPSP. RESULTS: A strong positive correlation was observed between the wavelet spectral entropy and other parameters such as amplitude, slope of rising phase and slope of descending phase of the EPSP. The paired-pulse facilitation (PPF) of the EPSP was significantly increased by rhein (30 micromol/L). The inhibitory postsynaptic potential (IPSP) recorded in the presence of CNQX (20 micromol/L) and APV (40 micromol/L) is not altered by rhein (30 micromol/L). CONCLUSIONS: Rhein (30 micromol/L) can decrease the frequency but not the amplitude of the miniature EPSP (mEPSP). It is suggested that rhein inhibits excitatory synaptic transmission by decreasing the release of glutamate in rat hippocampal CA1 pyramidal neurons.
Asunto(s)
Antraquinonas/farmacología , Entropía , Hipocampo/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Animales , Antraquinonas/química , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Hipocampo/fisiología , Técnicas In Vitro , Masculino , Ratas , Ratas WistarRESUMEN
Rhubarb extracts provide neuroprotection after brain injury, but the mechanism of this protective effect is not known. The present study tests the hypothesis that rhubarb extracts interfere with the release of glutamate by brain neurons and, therefore, reduce glutamate excitotoxicity. To this end, the effects of emodin, an anthraquinone derivative extracted from Rheum tanguticum Maxim. Ex. Balf, on the synaptic transmission of CA1 pyramidal neurons in rat hippocampus were studied in vitro. The excitatory postsynaptic potential (EPSP) was depressed by bath-application of emodin (0.3-30 microM). Paired-pulse facilitation (PPF) of the EPSP was significantly increased by emodin. The monosynaptic inhibitory postsynaptic potential (IPSP) recorded in the presence of glutamate receptor antagonists (DNQX and AP5) was not altered by emodin. Emodin decreased the frequency, but not the amplitude, of the miniature EPSP (mEPSP). The inhibition of the EPSP induced by emodin was blocked by either 8-CPT, an adenosine A1 receptor antagonist, or by adenosine deaminase. These results suggest that emodin inhibits the EPSP by decreasing the release of glutamate from Schaffer collateral/commissural terminals via the activation of adenosine A1 receptors in rat hippocampal CA1 area and that the neuroprotective effects of rhubarb extracts may result from decreased glutamate excitotoxicity.
Asunto(s)
Emodina/farmacología , Hipocampo/citología , Células Piramidales/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Adenosina/farmacología , Adenosina Desaminasa/farmacología , Animales , Bicuculina/análogos & derivados , Bicuculina/farmacología , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Electrofisiología/métodos , Inhibidores Enzimáticos/farmacología , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Técnicas In Vitro , Masculino , Inhibición Neural/efectos de los fármacos , Antagonistas de Receptores Purinérgicos P1 , Células Piramidales/fisiología , Quinoxalinas/farmacología , Ratas , Ratas Wistar , Transmisión Sináptica/fisiología , Teofilina/análogos & derivados , Teofilina/farmacología , Valina/análogos & derivados , Valina/farmacologíaRESUMEN
Effects of milnacipran (MIL), a selective serotonin and noradrenaline (NA) reuptake inhibitor, on the neuronal excitability and synaptic transmission in the rat locus coeruleus (LC) were examined by intracellular and whole-cell patch-clamp recording techniques. We compared MIL and methylphenidate (MPH), a selective NA and dopamine reuptake inhibitor, as a therapeutic agent for attention deficit/hyperactivity disorder. Application of MPH (1-100 microM) and MIL (1-100 microM) to artificial cerebrospinal fluid (ACSF) produced a hyperpolarizing response in LC neurons in a concentration-dependent manner. Spontaneous firing of LC neurons was blocked during the hyperpolarization. The MIL-induced hyperpolarization was blocked by yohimbine (1 microM), an antagonist for alpha-adrenoceptors. These results suggest that the MIL-induced hyperpolarization is mediated by NA via alpha2-adrenoceptors in LC neurons. Under the whole-cell patch-clamp condition, prolonged application of MIL produced an outward current which lasted as long as MIL existed in the ACSF. The outward current induced by NA was enhanced by MIL in LC neurons. MIL enhanced the amplitude and duration of the inhibitory postsynaptic potential, while it depressed the excitatory postsynaptic potential. The results indicated that both MIL and MPH showed almost the same effects on neuronal activity and synaptic transmission in the rat LC. These results suggest that MIL increases the concentration of NA at synaptic clefts by inhibiting the NA reuptake system in the rat LC.
Asunto(s)
Ciclopropanos/farmacología , Locus Coeruleus/efectos de los fármacos , Neuronas/fisiología , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología , Transmisión Sináptica/efectos de los fármacos , Animales , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Locus Coeruleus/citología , Masculino , Metilfenidato/farmacología , Milnaciprán , Neuronas/efectos de los fármacos , Norepinefrina/metabolismo , Norepinefrina/farmacología , Técnicas de Placa-Clamp , Ratas , Ratas WistarRESUMEN
OBJECTIVES: We sought to assess whether mechanical unloading has beneficial effects on cardiomyocytes from doxorubicin-induced cardiomyopathy in rats. BACKGROUND: Mechanical unloading by a left ventricular assist device (LVAD) improves the cardiac function of terminal heart failure in humans. However, previous animal studies have failed to demonstrate beneficial effects of mechanical unloading in the myocardium. METHODS: The effects of mechanical unloading by heterotopic abdominal heart transplantation were evaluated in the myocardium from doxorubicin-treated rats by analyzing the intracellular free calcium level ([Ca(2+)](i)) and the levels of intracellular Ca(2+)-regulatory proteins. RESULTS: In doxorubicin-treated rats, the duration of cell shortening and [Ca(2+)](i) transients in cardiomyocytes was prolonged (432 +/- 28.2% of control in 50% relaxation time; 184 +/- 10.5% of control in [Ca(2+)](i) 50% decay time). Such prolonged time courses significantly recovered after mechanical unloading (114 +/- 10.4% of control in 50% relaxation time; 114 +/- 5.8% of control in 50% decay time). These effects were accompanied by an increase in sarcoplasmic reticulum Ca(2+) ATPase (SERCA2a) protein levels (0.97 +/- 0.05 in unloaded hearts vs. 0.41+/- 0.09 in non-unloaded hearts). The levels of other intracellular Ca(2+)-regulatory proteins (phospholamban and ryanodine receptor) were not altered after mechanical unloading in doxorubicin-treated hearts. These parameters in unloaded hearts without doxorubicin treatment were similar to normal hearts. CONCLUSIONS: Mechanical unloading increases functional sarcoplasmic reticulum Ca(2+) ATPase and improves [Ca(2+)](i) handling and contractility in rats with doxorubicin-induced cardiomyopathy. These beneficial effects of mechanical unloading were not observed in normal hearts.
Asunto(s)
Calcio/fisiología , Cardiomiopatías/fisiopatología , Trasplante de Corazón/fisiología , Contracción Miocárdica/fisiología , Miocitos Cardíacos/fisiología , Animales , Antibióticos Antineoplásicos , ATPasas Transportadoras de Calcio/fisiología , Cardiomiopatías/inducido químicamente , Doxorrubicina , Miocardio/ultraestructura , Ratas , Ratas Endogámicas Lew , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Canal Liberador de Calcio Receptor de Rianodina/fisiología , Retículo Sarcoplasmático/metabolismo , ATPasas Transportadoras de Calcio del Retículo SarcoplásmicoRESUMEN
Effects of 5-hydroxytryptamine (5-HT) on inhibitory synaptic transmission in the rat dorsolateral septal nucleus (DLSN) were examined by conventional intracellular and voltage-clamp recording methods. 5-HT (1-30 microM) depressed the monosynaptic fast IPSC evoked by local stimulation of the DLSN in the presence of DNQX, AP5 and CGP 55845A. CP 93129, a selective 5-HT1B receptor agonist, depressed the fast IPSC. The 5-HT-induced depression of the fast IPSC was attenuated by SB 216641, a selective antagonist for 5-HT1B receptors. 5-HT did not change the inward currents mediated by GABAA receptors, suggesting that 5-HT presynaptically inhibited the fast IPSC. 5-HT and CP 93129 depressed the frequency of miniature fast IPSPs (mIPSPs) without changing their amplitudes. Neither a selective protein kinase A inhibitor, H-89, nor a selective protein kinase C inhibitor, calphostin C, blocked the 5-HT-induced depression of the fast IPSC. N-Ethylmaleimide (NEM) blocked the 5-HT-induced depression of the evoked IPSC. These results suggest that activation of presynaptic 5-HT1B receptors depresses the release of GABA via a pertussis toxin (PTX)-sensitive G-protein in the rat DLSN.
Asunto(s)
Inhibición Neural/fisiología , Neuronas/fisiología , Terminales Presinápticos/fisiología , Receptor de Serotonina 5-HT1B/fisiología , Núcleos Septales/citología , Análisis de Varianza , Animales , Benzamidas/farmacología , Compuestos de Bifenilo/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Inhibidores Enzimáticos/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Agonistas del GABA/farmacología , Antagonistas del GABA/farmacología , Técnicas In Vitro , Masculino , Potenciales de la Membrana/fisiología , Muscimol/farmacología , Oxadiazoles/farmacología , Técnicas de Placa-Clamp/métodos , Toxina del Pertussis/farmacología , Ácidos Fosfínicos/farmacología , Piperazinas/farmacología , Propanolaminas/farmacología , Piridinas/farmacología , Pirroles/farmacología , Quinoxalinas/farmacología , Ratas , Ratas Wistar , Serotonina/farmacología , Antagonistas de la Serotonina/farmacología , Agonistas de Receptores de Serotonina/farmacología , Transmisión Sináptica , Ácido gamma-Aminobutírico/farmacología , omega-Agatoxina IVA/farmacología , omega-Conotoxina GVIA/farmacologíaRESUMEN
Effects of milnacipran (MIL), a serotonin and noradrenaline reuptake inhibitor (SNRI), on synaptic transmission were examined in the rat locus coeruleus (LC). Bath-application of MIL produced a hyperpolarization associated with a decrease in input resistance of LC neurons. The MIL-induced hyperpolarization reversed polarity near the equilibrium potential of K+. The MIL-induced hyperpolarization was blocked by yohimbine (1 microM). Clonidine, but not serotonin (5-hydroxytryptamine; 5-HT), produced a hyperpolarizing potential in LC neurons. The MIL-induced hyperpolarization reversed polarity at -114 +/- 3 mV (n=4). MIL (0.1-10 microM) depressed the amplitude of the excitatory postsynaptic potential (EPSP), while it enhanced the amplitude and duration of the inhibitory postsynaptic potential (IPSP). These results suggest that MIL hyperpolarizes LC neurons and enhances the IPSP by increasing endogenous noradrenaline (NA) concentration at synapses in LC neurons.
Asunto(s)
Inhibidores de Captación Adrenérgica/farmacología , Ciclopropanos/farmacología , Locus Coeruleus/efectos de los fármacos , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología , Transmisión Sináptica/efectos de los fármacos , Animales , Locus Coeruleus/fisiología , Masculino , Potenciales de la Membrana/efectos de los fármacos , Milnaciprán , Ratas , Ratas Wistar , Serotonina/farmacologíaRESUMEN
Effects of L-arginine on the heat-induced depression of the neuronal activity in the hippocampal CA1 area were investigated using optical recording techniques. An increase in the temperature of hippocampal neurons from 32 degrees C to 38 degrees C reversibly depressed the fast and slow components of the optical response to stimulation of the Schaffer collaterals that correspond to the presynaptic action potential and excitatory postsynaptic response, respectively. The neuronal activity recovered almost completely after cooling the hippocampal neurons back to 32 degrees C. A temperature increase to 40 degrees C produced irreversible depression of the neuronal activity. Pyruvate, but not lactate, in the artificial cerebrospinal fluid (ACSF) attenuated the depression of the neuronal activity induced by a temperature increase to 38 degrees C. Bath-application of L-arginine (1 mM), a nitric oxide (NO) donor, enhanced the depression of the neuronal activity at 38 degrees C. In the presence of L-arginine, the recovery of neuronal activity, upon return to 32 degrees C, was incomplete. The contribution of NO to the heat-induced impairment of the neuronal activity was discussed.
Asunto(s)
Arginina/farmacología , Hipocampo/efectos de los fármacos , Calor , Neuronas/efectos de los fármacos , Animales , Hipocampo/citología , Hipocampo/metabolismo , Hipocampo/fisiología , Masculino , Neuronas/metabolismo , Neuronas/fisiología , Óxido Nítrico/biosíntesis , Ratas , Ratas WistarRESUMEN
Using optical recording techniques, we examined whether nitric oxide (NO) is implicated in the impairment of the activity of hippocampal CA1 neurons induced by mild heat stress. A temperature increase from 32 to 38 degrees C reversibly depressed the neuronal activity in hippocampal slices. L-Arginine (1 mM), an NO donor, enhanced the heat-induced depression of the activity of hippocampal CA1 neurons. N(omega)-Nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, attenuated the inhibition of the neuronal activity induced by a temperature increase. Methylcobalamin (10 microM), a vitamin B(12) analogue that reduces NO production, reduced the heat-induced depression of the neuronal activity. These results suggest that NO contributes, at least in part, to the heat-induced depression of the neuronal activity in the hippocampal CA1 region.
Asunto(s)
Hipocampo/fisiología , Neuronas/fisiología , Óxido Nítrico/fisiología , Vitamina B 12/análogos & derivados , Animales , Arginina/farmacología , Estimulación Eléctrica , Electrofisiología , Calefacción , Hipocampo/citología , Técnicas In Vitro , Masculino , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico/antagonistas & inhibidores , Donantes de Óxido Nítrico/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óptica y Fotónica , Ratas , Ratas Wistar , Temperatura , Vitamina B 12/farmacologíaRESUMEN
Using optical recording methods in the rat lateral septal nucleus (LSN) slice, we examined the question of whether antecedent hypoglycemia protects neurons from the adverse effects of subsequent hypoglycemic stimuli. The first exposure of LSN neurons to glucose deprivation for 15 min produced a marked depression of neuronal activity. The neuronal activity recovered by reapplication of glucose to the neurons. In neurons previously exposed to glucose deprivation, subsequent applications of glucose-free artificial cerebrospinal fluid (ACSF) produced only weak depression of the neuronal activity. The contribution of monocarboxylate transporters to this adaptation of neuronal activity to repeated glucose deprivation was examined in LSN neurons. alpha-Cyano-4-hydroxycinnamate (4-CIN, 100 microM), an inhibitor of the monocarboxylate transporters, did not significantly affect the depression of the neuronal activity induced by the first glucose deprivation. However, in the presence of 4-CIN (100 microM), a second glucose deprivation produced a typical depression of the neuronal activity, indicating that 4-CIN had nullified the adaptation of neuronal activity to a second glucose deprivation. Cytochalasin B (CCB, 20 microM), an inhibitor of glucose transporters, depressed the neuronal activity in the presence of 11 mM glucose. Pyruvate (11 mM) partially restored the neuronal activity depressed by pretreatment with CCB (20 microM) for 30-40 min. These results suggest that antecedent glucose deprivation stimulates monocarboxylate-transporters to supply energy substrates to LSN neurons, thus protecting the neurons against subsequent glucose deprivation. .
Asunto(s)
Adaptación Fisiológica/fisiología , Glucosa/deficiencia , Transportadores de Ácidos Monocarboxílicos/metabolismo , Neuronas/metabolismo , Núcleos Septales/metabolismo , Adaptación Fisiológica/efectos de los fármacos , Animales , Masculino , Neuronas/efectos de los fármacos , Ratas , Ratas Wistar , Núcleos Septales/efectos de los fármacosRESUMEN
Alterations in intracellular Ca2+ ([Ca2+]i) are generally associated with cellular distress. Oxalate-induced cell injury of the renal epithelium plays an important role in promoting CaOx nephrolithiasis. However, the degree of change in intracellular free calcium ions in renal epithelial cells during oxalate exposure remains unclear. The aim of this study is to determine whether acute short-term exposure to oxalate produces morphological changes in the cells, induces a change in cytosolic Ca2+ levels in renal tubular epithelial cells and whether the application of extracellular glycosaminoglycans (GAGs) prevents these changes. Cultured Mardin-Darby canine kidney cells were exposed to oxalate, and changes in cytosolic Ca2+ were determined under various conditions. The effect of heparin and heparan sulfate (HS) during oxalate exposure was examined. The change in the GAG contents of the culture medium was also determined. Transmission electron microscopy (TEM) was performed for morphological analysis. The degree of change in cytosolic Ca2+ strongly correlated with oxalate concentration. Cytosolic Ca2+ levels decreased in parallel with an increase in the concentration of oxalate. However, this decrease was strongly inhibited by pretreatment with heparin or HS. TEM revealed cytoplasmic vacuolization, the appearance of flocculent material and mitochondrial damage after oxalate exposure. On the other hand, pretreatment with heparin or HS completely blocked these morphological changes. The present data suggest that acute exposure to a high concentration of oxalate challenges the renal cells, diminishes their viability and induces changes in cytosolic Ca2+ levels. Heparin and HS, which are known as potent inhibitors of CaOx crystallization, may also prevent oxalate-induced cell changes by stabilizing the cytosolic Ca2+ level.
Asunto(s)
Oxalato de Calcio/farmacología , Calcio/metabolismo , Citoprotección , Heparina/farmacología , Heparitina Sulfato/farmacología , Membranas Intracelulares/metabolismo , Riñón/metabolismo , Riñón/patología , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Perros , Líquido Extracelular/metabolismo , Riñón/efectos de los fármacos , Riñón/fisiopatología , Cálculos Renales/prevención & control , Microscopía ElectrónicaRESUMEN
Effects of 5-hydroxytryptamine (5-HT) on EPSPs and EPSCs in the rat dorsolateral septal nucleus (DLSN) were examined in the presence of GABA(A) and GABA(B) receptor antagonists. Bath application of 5-HT (10 microm) for 5-10 min increased the amplitude of the EPSP and EPSC. (+/-)-8-hydroxy-2-(di-N-propylamino)tetralin hydrobromide (10 microm), an agonist for 5-HT1A and 5-HT7 receptors, did not facilitate the EPSP. alpha-Methyl-5-HT (10 microm), a 5-HT2 receptor agonist, increased the amplitude of the EPSC. Alpha-methyl-5-(2-thienylmethoxy)-1H-indole-3-ethanamine (10 microm) and 6-chloro-2-(1-piperazinyl)pyrazine (10 microm), selective 5-HT2B and 5-HT2C receptor agonists, respectively, had no effect on the EPSP. The 5-HT-induced facilitation of the EPSP was blocked by ketanserin (10 microm), a 5-HT2A/2C receptor antagonist. However, N-desmethylclozapine (10 microm), a selective 5-HT2C receptor antagonist, did not block the facilitation of the EPSP induced by alpha-methyl-5-HT. The inward current evoked by exogenous glutamate was unaffected by 5-HT. 5-HT (10 microm) and alpha-methyl-5-HT (10 microm) increased the frequency of miniature EPSPs (mEPSPs) without changing the mEPSP amplitude. The ratio of the paired pulse facilitation was significantly decreased by 5-HT and alpha-methyl-5-HT. The 5-HT-induced facilitation of the EPSP was blocked by calphostin C (100 nm), a specific protein kinase C (PKC) inhibitor, but not by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (10 microm), a protein kinase A inhibitor. Phorbol 12,13-dibutyrate (3 microm) mimicked the facilitatory effects of 5-HT. These results suggest that 5-HT enhances the EPSP by increasing the release of glutamate via presynaptic 5-HT2A receptors that link with PKC in rat DLSN neurons.
Asunto(s)
Terminales Presinápticos/metabolismo , Proteína Quinasa C/metabolismo , Receptores de Serotonina/metabolismo , Núcleos Septales/metabolismo , Transmisión Sináptica/fisiología , Animales , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Antagonistas del GABA/farmacología , Antagonistas de Receptores de GABA-A , Antagonistas de Receptores de GABA-B , Proteínas de Unión al GTP/efectos de los fármacos , Proteínas de Unión al GTP/metabolismo , Ácido Glutámico/farmacología , Técnicas In Vitro , Masculino , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Proteína Quinasa C/antagonistas & inhibidores , Ratas , Ratas Wistar , Receptor de Serotonina 5-HT2A , Núcleos Septales/citología , Núcleos Septales/efectos de los fármacos , Serotonina/farmacología , Antagonistas de la Serotonina/farmacología , Agonistas de Receptores de Serotonina/farmacología , Transmisión Sináptica/efectos de los fármacos , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
Effects of fluid percussion injury (FPI) of the parietal cerebral cortex on the neuronal activity in the temporal region of the rat hippocampal CA1 area were investigated by using optical and extracellular recording techniques. Application of moderate impact (1.5-2.0 atm) to the parietal cerebral cortex enhanced the optical signal of the neuronal activity in the ipsilateral hippocampal CA1 area. The field potential evoked by the Schaffer collaterals had multiple population spikes in the ipsilateral hippocampal CA1 pyramidal cell layer. Bicuculline (15 microM) increased the amplitude and the number of population spikes of the field potential even after the brain injury. These results suggest that FPI produces hyperexcitability of hippocampal CA1 neurons, probably by increasing the activity of the Schaffer collaterals of hippocampal CA3 neurons.