RESUMEN

OBJECT: The incidence of carotid artery dissection related to blunt injury is very low, but the mortality rate is high. Rapid diagnosis and proper treatments are discussed. CLINICAL PRESENTATION: A 48-year-old woman presented diplopia and pulsating tinnitus of the left ear. An angiography showed a carotid cavernous fistula (CCF) and dissection of the extra-cranial internal carotid artery (ICA). To treat the dissection, a self-expanding endovascular stent was used. She has been followed for 6 years without any event and the ICA is patent. CONCLUSION: Prompt diagnosis without delay and intimate follow-up is the key for the treatment of a carotid injury. Those patients who exhibit cervical bruits and/or seat-belt signs should be examined aggressively. Angioplasty with stents is amenable for patients with traumatic carotid dissections requiring vascular reconstruction in the acute stage.

Asunto(s)

Disección de la Arteria Carótida Interna/cirugía , Fístula del Seno Cavernoso de la Carótida/cirugía , Cinturones de Seguridad/efectos adversos , Stents , Procedimientos Quirúrgicos Vasculares/instrumentación , Accidentes de Tránsito , Arteria Carótida Interna/diagnóstico por imagen , Arteria Carótida Interna/patología , Arteria Carótida Interna/cirugía , Disección de la Arteria Carótida Interna/etiología , Disección de la Arteria Carótida Interna/patología , Fístula del Seno Cavernoso de la Carótida/etiología , Fístula del Seno Cavernoso de la Carótida/patología , Angiografía Cerebral , Circulación Cerebrovascular/fisiología , Femenino , Movimientos de la Cabeza/fisiología , Humanos , Persona de Mediana Edad , Resultado del Tratamiento , Procedimientos Quirúrgicos Vasculares/métodos , Heridas no Penetrantes

RESUMEN

SUMMARY: We reviewed the cranial nerve dysfunctions of eight patients with symptomatic cavernous internal carotid (CSIC) aneurysms treated by endovascular intraaneurysmal occlusion. Aneurysms were classified into three types according to their location and direction of growth. Anterior type aneurysms, which involved anterior bend of CSIC represented third nerve dysfunction. Posterior type aneurysms, which located posterior bend of CSIC preferred to affect sixth nerve function. CSIC aneurysms that extended over the both bends had total ophthalmoplegia. All patients responded to endovascular treatment, though partial resolution was recorded in the case of upward gaze or lateral gaze impairment. Endovascular treatment with detachable coils offers an excellent alternative with acceptable risks of morbidity.

RESUMEN

Intracranial dissecting aneurysm (DA) is much less frequent than berry aneurysm. Such dissection involves mostly the vertebral and basilar arteries, followed by the internal carotid and middle cerebral arteries. DA of the anterior cerebral artery (ACA) is relatively rare and little is known about its natural Development. Only 23 cases have been reported previously. Our present patient, a 44-year-old man, suddenly developed paresis of the left leg while bathing. Diffusion magnetic resonance imaging indicated an area of high signal intensity in the territory of the ACA. Angiography on day 3 following onset showed a DA involving the left A2 segment. Antiplatelet therapy was administered. Further luminal narrowing in the lesion was demonstrated by repeat angiography on day 17. Occlusion of the distal A2 segment was demonstrated together with sufficient collateral supply on day 41. Symptoms resolved completely. DA of the ACA usually presents with ischemic attacks. Its etiology remains uncertain, and its natural course is unclear. Surgical intervention is recommended for patients with intracranial hemorrhage, while non surgical therapies have achieved good outcomes in ischemic cases.

Asunto(s)

Disección Aórtica/fisiopatología , Circulación Cerebrovascular , Circulación Colateral , Aneurisma Intracraneal/fisiopatología , Adulto , Disección Aórtica/diagnóstico por imagen , Disección Aórtica/patología , Angiografía Cerebral , Humanos , Aneurisma Intracraneal/diagnóstico por imagen , Aneurisma Intracraneal/patología , Imagen por Resonancia Magnética , Masculino

RESUMEN

We described a dissecting aneurysm of the vertebral artery (VA), which was associated with neurofibromatosis type 1 (NF1). A 41-year-old man was referred to our hospital because of abrupt, severe headache. A CT scan revealed diffuse subarachnoid hemorrhage (SAH) predominantly in the prepontine cistern. The angiograms showed a string sign in the left VA, just distal to the posterior inferior cerebellar artery (PICA). The vertebral dissection was considered responsible for SAH, and endovascular occlusion of the left VA was attempted. During the intervention, the patient complained of severe neck pain at the time of selective vertebral angiography, which revealed an arteriovenous fistula. The VA was occluded proximal to the PICA with GDC, which covered the fistula. Open surgery confirmed the two unruptured aneurysms. Intracranial dissection is rarely reported in association with NF1. However, ateriovenous fistula is not an uncommon combination with dissecting aneurysm and the extracranial segment of the VA is a characteristic target. Anatomical feasibility is conceivably the pathogenesis.

Asunto(s)

Disección Aórtica/complicaciones , Fístula Arteriovenosa/etiología , Neurofibromatosis 1/complicaciones , Hemorragia Subaracnoidea/complicaciones , Arteria Vertebral , Adulto , Humanos , Masculino

RESUMEN

We reported a case of peduncular hallucination after aneurysmal subarachnoid hemorrhage (SAH). The patient underwent endovascular embolization of an intracranial aneurysm using the Guglielmi detachable coils (GDCs) 9 days after SAH. On the 11th day, she reported visual hallucination: a maggot was on the ceiling, or a soldier who wore green clothes was standing by a locker. The hallucination was vivid, well-formed and associated with insomnia, suggesting peduncular hallucination. A computed tomographic (CT) scan revealed small infarctions of right frontal lobe, which were not responsible for the visual hallucination. Hyperdynamic therapy relieved the visual hallucination 23 days after SAH. It was conceivable that vasospasm was the cause of the infarction and visual hallucination. Only 4 cases with peduncular hallucination after SAH were reported in conjunction with vasospasm. The symptom may be concealed by disturbance of consciousness. Visual hallucination should be considered as a sign of cerebral vasospasm, and an appropriate treatment should be done at right time.

Asunto(s)

Alucinaciones/etiología , Hemorragia Subaracnoidea/complicaciones , Anciano , Femenino , Humanos , Aneurisma Intracraneal/complicaciones , Ataque Isquémico Transitorio/complicaciones

RESUMEN

Several pathological conditions are responsible for abducens palsy, but a lesion of the vertebral artery (VA) has rarely been recognized as one of the causes. It has been reported that a high percentage of cases of abducens palsy are involved with ruptured dissecting aneurysms of the VA. We investigate the vertebrobasilar anatomy in 4 patients, suffering with abducens palsy. One patient revealed a fusiform dilatation of the vertebral artery on the same side as the abducens palsy. Three patients were noted to have stenosis of the vertebral artery from the origin of the posterior inferior cerebellar artery (PICA) to the union. Finally, four patients were found to have abnormal vertebrobasilar anatomy, which was on the same side as the abducens palsy. It is conceivable that changes of vertebrobasilar circulation may cause abducens palsy through direct compression, or ischemic events.

Asunto(s)

Nervio Abducens , Aneurisma Roto/complicaciones , Disección Aórtica/complicaciones , Enfermedades de los Nervios Craneales/etiología , Parálisis/etiología , Arteria Vertebral , Insuficiencia Vertebrobasilar/complicaciones , Adulto , Anciano , Constricción Patológica , Femenino , Humanos , Masculino , Persona de Mediana Edad

RESUMEN

We undertook stent-supported angioplasty for 35 lesions of extracranial stenosis in 31 patients from May 1996 to October 1998. We succeeded in the stenting at the predetermined sites of all lesions and excellent initial results were obtained. The mean % stenosis decreased from 81% to 7.1% after stenting. Four patients had neurological complications related to the procedure. There were two transient ischemic attacks (one in carotid and one in vertebral stenting), and permanent deficits occurred in two patients treated for carotid stenosis. In carotid stenting, we observed long-term patency for over six months in all 17 patients (18 lesions). Mean angiographic, asymptomatic restenosis was 20% and 21% at three and six months, respectively (range, 4 to 38%). No further stenosis was observed thereafter. Deformity of the stents were not noted in any patient. In vertebral stenting, four out of nine cases revealed significant restenosis between three and six months later, while these were dilated by repeated PTA. On strict definition of indication or application of a cerebral protection, stent-supported angioplasty is an effective method for the treatment of extracranial stenotic lesions.

RESUMEN

The light and electron microscopic localizations of single stranded DNA (SSD) protein, a marker of apoptosis and programmed cell death, in the gerbil hippocampus were examined by immunocytochemistry after transient brain ischemia. SSD-immunoreactive (IR) cells appeared from post-operative day 1 (PO 1) to PO 7 after 5- or 10-min ischemia. Immunoreaction was recognized in the nucleus of the CA1 pyramidal neurons without remarkable morphological changes on PO 1. These findings suggest that SSD degradation can occur during delayed neuronal death in the CA1, preceding the appearance of double strand breaks, one of the characteristic features of apoptosis.

Asunto(s)

Apoptosis/genética , Isquemia Encefálica/fisiopatología , ADN de Cadena Simple/análisis , Proteínas de Unión al ADN/análisis , Hipocampo/fisiopatología , Ribonucleoproteínas/análisis , Animales , Biomarcadores , Isquemia Encefálica/metabolismo , Femenino , Gerbillinae , Hipocampo/química , Hipocampo/ultraestructura , Inmunohistoquímica , Masculino

RESUMEN

SUMMARY: The results of nine patients with carotid stenosis in a high-risk surgical population that were treated by stent supported angioplasty are reported. There were eight males and one female between the ages of 53 to 74. A balloon expandable stent was deployed by a transfemoral approach. Technical success was achieved in all cases. There were no periprocedural complications. The mean % stenosis decreased from 84% to 5.2% after stenting. No arterial dissection was recorded and smooth contour of the vessel was demonstrated in all patients. We have observed long term patency for over 6 months in seven patients. Mean angiographic stenosis was 20% and 21% at 3 and 6 months, respectively (range, 5 to 32%). No further stenosis was recorded. Carotid stenting is an alternative strategy to carotid endoarterectomy (CEA) for high-risk patients, for whom the complications of CEA may exceed the potential benefits.

RESUMEN

The relationship between extravasation of serum albumin and damage to the neuronal elements as well as the astrocytic reaction was investigated following cold injury, using immunohistochemistry for albumin, microtubule-associated protein I and II (MAPs) and glial fibrillary acidic protein (GFAP). After 30 min, spreading of albumin to the neuropil and uptake into nerve cell bodies and dendrites were clearly observed in the area surrounding the cold lesion. Extravasation of albumin was maximal at 24 h and extended to the ipsilateral hippocampus and thalamus as well as to the paramedian part of the contralateral cerebral hemisphere. Uptake of albumin was seen in neurons with and without loss or reduction of the reaction for MAPs, but the former was confined to the area surrounding the cold lesion. When extravasated albumin receded from the neuropil, the positive reaction for albumin also disappeared from the neuronal elements and those neurons recovered immunoreactivity for MAPs. Astrocytes immunopositive for albumin were observed at 24 h in the white matter, and reactive astrocytes became notable even in the gray matter surrounding the cold lesion. Although reactive astrocytes persisted even after resolution of cerebral edema, immunopositivity for albumin disappeared from astrocytes soon after the disappearance of the reaction from the neuropil. As to the mechanism, rapid endo- and exocytosis may take place in response to the amount of edema fluid in the surrounding extracellular space, where albumin may be eliminated through the transvascular route and/or via the cerebrospinal fluid space.

Asunto(s)

Astrocitos/fisiología , Frío/efectos adversos , Neuronas/fisiología , Animales , Astrocitos/química , Astrocitos/citología , Edema Encefálico/metabolismo , Edema Encefálico/patología , Lesiones Encefálicas/etiología , Gerbillinae , Proteína Ácida Fibrilar de la Glía/fisiología , Inmunohistoquímica/métodos , Proteínas Asociadas a Microtúbulos/fisiología , Neuronas/química , Neuronas/citología , Neutrófilos/fisiología , Albúmina Sérica/fisiología

RESUMEN

A 51-year-old man presented with headache, vomiting and exophthalmus. Neurological examination revealed anosmia, papilledema, decrease in visual acuity, and disability in ocular movement. MRI showed a huge mass which occupied the whole nasal cavity and compressed the frontal lobe upwards and the eyes laterally. CT revealed an extensive bony destruction of the frontal base and bilateral orbits. The mass was biopsied transnasally, and was histologically diagnosed as olfactory neuroblastoma. It was highly radiosensitive and disappeared with a local irradiation of 40 Gy. Three months later the patient complained of a pain radiating from the neck to the right arm. MRI demonstrated a metastasis at the vertebral body of C5. Local irradiation of 30 Gy was performed. The metastatic lesion was removed, and a bone graft taken from the iliac bone was transplanted via an anterior cervical approach. Three weeks later, however, a hard mass appeared in the right of his neck and was surgically removed. By histological examination, it was also identified as a metastatic neuroblastoma to the cervical lymph node. A week after the removal of the cervical metastatic lesion, the metastasis extended rapidly to the left cervical and the bilateral hilar lymph nodes of the lungs. Chemotherapy was performed with a total doses of 800mg of cyclophosphamide, 1.5mg of vincristine, 40mg of pirarubicin, and 80mg of cisplatin. The lesions disappeared within 7 days. However, the patient died from disseminated intravascular coagulation 10 months after the onset. Olfactory neuroblastoma is usually an intranasal neoplasm, but it rarely extends intracranially and intraorbitally as is shown in our case. Basically, olfactory neuroblastoma is a relatively slow-growing tumor though it has a tendency to develop local recurrences over long periods even after aggressive primary treatment, and accompanied with distant metastases. However, our patient showed a very short survival time. Invasive extension and multiple metastases occurred during a short period, followed by disseminated intravascular coagulation. Combined chemotherapy at the initial treatment may be recommended in such an extensive case.

Asunto(s)

Neoplasias Encefálicas/patología , Neoplasias Encefálicas/secundario , Neuroblastoma/secundario , Vías Olfatorias , Neoplasias Orbitales/secundario , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Neoplasias Encefálicas/tratamiento farmacológico , Humanos , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Neuroblastoma/tratamiento farmacológico

RESUMEN

SUMMARY: We evaluated the feasibility of stenting in four patients. Two cases were vertebral osteal stenosis and the others were carotid stenosis at high position with ulcers. We placed balloon expandable coronary stents by a bared stent technique. The mean preprocedural stenosis (86.58%) was reduced to 13.05%. Patients were examined clinically and angiographically at 1,3 and 6 months after stenting. There was no minor nor major stroke during and after the procedures. Asymptomatic restenosis occurred in the cases of proximal vertebral arteries. One of these patients needed to repeat balloon dilatation. There were no angiographic restenoses in the location of stenting in the carotid artery. In the treatment for atherosclerotic stenoses, stent placement is a feasible and safe method. However restenosis in a vertebral osteal lesion should be carefully followed after stent placement.

RESUMEN

A 42-year-old male presented with the complaint of mild left facial numbness. Magnetic resonance imaging demonstrated a solid tumor in the interpeduncular cistern and a huge arachnoid cyst in the left middle cranial fossa. The tumor appeared isointense to the surrounding cerebral gray matter on T1-weighted images and hyperintense to that on T2-weighted images. The tumor was partially resected. Histological findings were characteristic of hamartoma. The mild left facial numbness was probably due to compression of the left trigeminal nerve by the arachnoid cyst. Asymptomatic hypothalamic hamartomas may occur in adults with atypical clinical presentations.

Asunto(s)

Quistes Aracnoideos/complicaciones , Hamartoma/complicaciones , Enfermedades Hipotalámicas/complicaciones , Adulto , Quistes Aracnoideos/diagnóstico , Quistes Aracnoideos/cirugía , Coristoma/diagnóstico , Craneotomía , Diagnóstico Diferencial , Drenaje , Hamartoma/diagnóstico , Hamartoma/cirugía , Humanos , Enfermedades Hipotalámicas/diagnóstico , Enfermedades Hipotalámicas/cirugía , Imagen por Resonancia Magnética , Masculino , Neuroglía/patología

RESUMEN

We experienced that therapeutic embolization of a large cerebral arteriovenous malformation (AVM) led to venous outflow obstruction resulting in intracranial hypertension in a patient who had undergone external decompression. To evaluate hemodynamic changes after embolization, we monitored the cerebrospinal fluid pressure in the next four patients who underwent endovascular treatment. The embolization of a medium AVM resulted in a slight increase in the cerebrospinal fluid pressure. In two medium AVMs, embolization produced slight decreases in the cerebrospinal fluid pressure. In a small AVM, we did not observe any changes in the cerebrospinal fluid pressure during the endovascular treatment. We discuss the mechanism of changes in the intracranial pressure after embolization and conclude that monitoring of the cerebrospinal fluid pressure immediately yields useful information for hemodynamic changes during endovascular treatment.

Asunto(s)

Presión del Líquido Cefalorraquídeo/fisiología , Embolización Terapéutica , Malformaciones Arteriovenosas Intracraneales/líquido cefalorraquídeo , Malformaciones Arteriovenosas Intracraneales/terapia , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Monitoreo Fisiológico

RESUMEN

This report provides findings of an investigation of the influence of traumatic subarachnoid hemorrhage on the development of delayed cerebral ischemia caused by vasospasm. The authors prospectively studied 130 patients with closed-head trauma, who exhibited subarachnoid blood on admission computerized tomography (CT) scans. Ten (7.7%) of these patients developed delayed ischemic symptoms between Days 4 and 16 after the head injury. They consisted of three (3.0%) of 101 patients with small amounts of subarachnoid blood and seven (24.1%) of 29 patients with massive quantities of subarachnoid blood on admission CT scans. In each of the 10 patients, severe vasospasm was demonstrated by angiography performed soon after development of ischemic symptoms. There was a close correlation between the main site of the subarachnoid blood and the location of severe vasospasm. In seven of the patients, follow-up CT scans showed development of focal ischemic areas in the cerebral territories corresponding to the vasospastic arteries. These results demonstrate that traumatic subarachnoid hemorrhage, especially if massive, is a predictable indicator of delayed ischemic symptoms.

Asunto(s)

Lesiones Encefálicas/diagnóstico por imagen , Isquemia Encefálica/etiología , Hemorragia Subaracnoidea/diagnóstico por imagen , Adulto , Anciano , Angiografía Cerebral , Femenino , Humanos , Masculino , Persona de Mediana Edad , Hemorragia Subaracnoidea/complicaciones , Factores de Tiempo , Tomografía Computarizada por Rayos X

RESUMEN

A new animal model of cerebral infarction was developed by magnetic embolization with carbonyl iron particles. An electromagnet was placed upon the lateral portion outside the orbit of Mongolian gerbils and charged with a current intensity of 2 A, 3 V (400 G) for 10 min. By intracardiac injection of iron particles at the beginning of the electric charge, infarction was selectively produced in the cerebral cortex. Two days after the operation, microhemorrhaging was seen in the necrotic region. Multiple scattered infarctions accompanied with microhemorrhagic signs closely resembled embolic infarction encountered in the human brain.

Asunto(s)

Corteza Cerebral/patología , Infarto Cerebral/patología , Modelos Animales de Enfermedad , Fenómenos Electromagnéticos , Animales , Gerbillinae , Hierro , Factores de Tiempo

RESUMEN

Haemorrhage in regions remote from the site of following intracranial operations is rare, but they do occur. We performed supratentorial craniotomy on 639 patients between the time of introduction of computed tomography (CT) for clinical use in 1983 and June 1992; subarachnoid haemorrhage (SAH) in the posterior fossa occurred postoperatively in six of these cases. These included four patients with tumours in the sellar region, one with an arteriovenous malformation (AVM) and one who underwent superficial temporal artery (STA)-middle cerebral artery (MCA) anastomosis. The ages of the six patients ranged from 17-72 years. Haemorrhage occurred on the day of operation in one case and was detected on CT examination on the day following surgery in the remaining five cases. Of three patients with disturbance of consciousness, two underwent suboccipital craniectomy for reduction of intracranial pressure, while one received barbiturate therapy and later underwent cerebrospinal fluid (CSF) shunt surgery. No special treatment was necessary for the remaining three patients with less serious lesions. Five of the six patients ultimately recovered their pre-operative neurological status apart from the primary diseases. Factors inducing such haemorrhages seem likely to include displacement of the cerebellum by reduced CSF pressure during and after operations, and stretching and tearing of the veins and venules in the sulci of the tentorial surface of the cerebellum. Consideration should therefore be given to the maintenance of an appropriate CSF pressure during operation; this is particularly important in elderly patients and those with an atrophied cerebral cortex.

Asunto(s)

Encefalopatías/cirugía , Complicaciones Posoperatorias/cirugía , Hemorragia Subaracnoidea/cirugía , Neoplasias Supratentoriales/cirugía , Adolescente , Anciano , Encefalopatías/diagnóstico , Estenosis Carotídea/diagnóstico , Estenosis Carotídea/cirugía , Craneofaringioma/diagnóstico , Craneofaringioma/cirugía , Craneotomía , Femenino , Humanos , Malformaciones Arteriovenosas Intracraneales/diagnóstico , Malformaciones Arteriovenosas Intracraneales/cirugía , Masculino , Neoplasias Meníngeas/diagnóstico , Neoplasias Meníngeas/cirugía , Meningioma/diagnóstico , Meningioma/cirugía , Persona de Mediana Edad , Examen Neurológico , Neoplasias Hipofisarias/diagnóstico , Neoplasias Hipofisarias/cirugía , Complicaciones Posoperatorias/diagnóstico , Reoperación , Factores de Riesgo , Hemorragia Subaracnoidea/diagnóstico , Neoplasias Supratentoriales/diagnóstico , Tomografía Computarizada por Rayos X

RESUMEN

For patients with nonresectable glioblastoma (GB) or recurrent GB, we have recently been using an interstitial chemotherapy with biodegradable polylactic acid pellets containing nimustine chloride (ACNU), in combination with superselective arterial ACNU injection, routine irradiation and chemotherapy. The ACNU pellets are prepared by mixing polylactic acid powder and ACNU, and then melting the mixture at low temperature and moulding it into a thin pellet. Pharmacological anticancer activity was experimentally demonstrated by the finding that a region of suppression was present surrounding an ACNU pellet placed in a B6 melanoma cell culture disc, but that no such suppression was present around a control pellet. In order to determine the spatial and temporal distribution of ACNU, a small pellet (ACNU: 0.6 mg) was implanted in the frontal lobe of rats. ACNU concentration determined by HPLC was 61.0 micrograms/g brain tissue on day 1. 22.5 on day 3, and 5.5 on day 7; small amounts of ACNU were in fact released for at least 4 weeks after implantation. This pellet was used for the clinical treatment of 11 GB patients. Four patients had several pieces of pellets implanted immediately after CT-guided stereotactic biopsy, and the other 7 had pellets placed in residual tumor after partial removal at craniotomy. No ACNU was detectable in serum. CT studies obtained at subsequent appropriate intervals disclosed gas formation around the pellets, a slight increase in edema, and necrosis or decrease in CT enhancement of tumor beginning around day 12 after implantation. Bone marrow suppression did not occur, since ACNU was administered interstitially and in the range of 50-200 mg (average: 126 mg) per patient.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Neoplasias Encefálicas/tratamiento farmacológico , Glioblastoma/tratamiento farmacológico , Ácido Láctico , Nimustina/administración & dosificación , Adulto , Anciano , Animales , Biodegradación Ambiental , Implantes de Medicamentos , Femenino , Humanos , Lactatos/metabolismo , Masculino , Persona de Mediana Edad , Poliésteres , Polímeros/metabolismo , Ratas , Ratas Wistar

RESUMEN

The amounts of myocardial metallothionein (MT) and heavy metal (Zn, Cu) levels during the early stage of the experimental myocardial infarction model induced by isoproterenol (Isp) administration were measured by an atomic absorption spectrophotometry. MT was measured by the Cd-hem method. Myocardial infarction was induced by the administration of 75 mg/kg i.p. of Isp to rats weighing 270 +/- 10 g. Thirty minutes after Isp injection, Zn and Cu levels began to decrease and 12 h later, reached the minimal values compared with the control value. The level of MT began to increase 3 h after the Isp injection and reached the maximal value at 12 h, although MT remained undetectable in the control myocardial tissue by the Cd-hem method. MT levels in the liver increased and total Zn and Cu were elevated compared with the control value 12 h after Isp administration. These results suggest that MT is produced in the myocardium after Isp administration, and that the roles of MT in the heart and the liver are different. It was thought that a rise in MT was induced for the protection of the myocardial cells to injury.

Asunto(s)

Isoproterenol , Metalotioneína/metabolismo , Infarto del Miocardio/metabolismo , Miocardio/metabolismo , Animales , Cobre/metabolismo , Masculino , Infarto del Miocardio/inducido químicamente , Ratas , Ratas Wistar , Zinc/metabolismo

RESUMEN

Using normal human thyroid cells and tumor cells, the reconstruction of various diseased cells in collagen gel as well as the relationship between the morphology of colonies in collagen-embedded culture and the biological behavior (benignity, malignancy, metastasis, and invasion) of the original tumors were studied. In collagen gel culture, normal thyroid cells reorganized follicle-like constructions, and follicular adenoma cells showed in vivo-like constructions. However, two different types of colonies were observed in cultures of cells from papillary carcinomas. One was the branching type with many outgrowths projecting to three dimensions and the other was the spherical type without any outgrowths. These spherical colonies were observed in all cases of papillary carcinoma, but varied from one case to another. Metastasis and invasion were detected during pathological examination in cases with a high ratio of spherical colonies. Our results indicate that cells from highly metastatic and invasive thyroid cancer form spherical colonies in the collagen gel culture, and that this collagen culture is a useful method for studying the heterogeneity of tumor cells as well as the metastasis and invasive ability of tumor cells in vitro.

Asunto(s)

Adenoma/patología , Carcinoma Papilar/patología , Enfermedad de Graves/patología , Neoplasias de la Tiroides/patología , Adulto , Anciano , Células Cultivadas , Colágeno , Femenino , Geles , Humanos , Persona de Mediana Edad , Invasividad Neoplásica , Metástasis de la Neoplasia , Células Tumorales Cultivadas