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Objective @#To investigate the status quo of drug resistance and influencing factors of tuberculosis in Inner Mongolia , and to provide reference for accurate prevention and control of drug - resistant tuberculosis .@*Methods @#Random sampling was used in this study . TB patients from Tuberculosis designated hospital in Inner Mongolia were included , according to the rules and drug - resistant strains were identified and tested according to relevant norms . Composition ratio or rate was calculated for statistical description , and Logistic regression model was used to analyze the influencing factors of drug resistance in TB patients .@*Results @#Among 1321 patients, there were 936 males and 385 females, with an average age of (52.65 ±18 .09) years . The rates of mono - resistant , multidrug - resistant (MDR) , extensively drug - resistant (XDR) and total drug resistance were 19.00% , 11.58% , 11.66% and 42.24% , respectively . The highest resistance rates were ob served for streptomycin ( 7.27% ) , isoniazid (4.69% ) , and isoniazid + streptomycin (4.47% ) . The drug resistance spectrum presented diversity and com plexity . Compared to females , males had a higher proportion of drug resistance , and the difference was statistically significant (P < 0.001) . The proportion of patients who were sensitive to anti - tuberculosis drugs increased with age (P < 0.05) . Among different age groups , the proportion of drug - resistant patients was higher in the 20 - 40 age group , 40 - 60 age group , and 60 and above age group compared to the 0 - 20 age group (P < 0.05) . Addi tionally , the proportion of drug - resistant patients was higher in the 20 - 40 age group and 40 - 60 age group com pared to the 60 and above age group (P < 0.05) . Moreover , the proportion of drug - resistant and multi - drug resistant patients was higher among patients undergoing retreatment compared to those undergoing initial treatment (P < 0.001) . Multivariate Logistic regression analysis showed that male gender (OR = 1.48 , 95% CI: 1.02 - 2.14) , age 20 - 40 years (OR = 2.64 , 95% CI: 1.05 - 6.60) , retreatment (OR = 2.34 , 95% CI: 1.70 - 3.22) , and outpatient follow-up (OR = 1.56 , 95% CI: 1.05 - 2.33) were independent risk factors for drug - resistant tuber culosis .@*Conclusion @#Inner Mongolia has a high prevalence of MDR and overall drug - resistant tuberculosis among patients . The drug resistance profile exhibits diversity and complexity . Risk factors that contribute to drug resistance include being male , aged between 20 and 40 , undergoing retreatment , and receiving outpatient follow - up . Therefore , it is necessary to further improve clinical diagnosis and treatment , promote rational use of first - line anti - tuberculosis drugs , prioritize individualized treatment , enhance health education , improve the medical insurance system , and optimize patient management approaches in order to enhance patient compliance .
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Objective @#To investigate the nutritional status and dietary structure of tuberculosis patients among different populations , analyze the factors influencing the nutritional status of tuberculosis patients , and provide theoretical basis for improving clinical nutrition and related issues in tuberculosis patients.@*Methods @#Tuberculosis patients , non⁃tuberculosis patients , and healthy individuals were randomly selected for a questionnaire survey. Descriptive analysis was conducted using SPSS 20. 0 software. Statistical description was performed using rates and composition ratios , and qualitative data were described using relative numbers. Chi⁃square test was used to compare overall rates and composition ratios among different health conditions groups , with a significance level of α = 0. 05. Independent factors analysis of nutritional status body mass index (BMI) was conducted using multiple Logistic regression analysis for variables with statistically significant differences in the univariate analysis. @*Results @#There were differences in the nutritional status (χ2 = 62. 184 , P < 0. 05) and dietary diversity score (χ2 = 64. 049 , P < 0. 05 ) among tuberculosis patients , non⁃tuberculosis patients , and healthy individuals. Univariate analysis of nutritional status BMI showed statistically significant differences in gender, smoking , meat⁃based diet , vegetable⁃based diet , moderate diet diversity score , and 6 other variables for tuberculosis patients ( P < 0. 05 ) , and in gender, age , ethnicity , marital status , occupation , education level , smoking , drinking white wine , drinking beer, meatcally significant differences in the univariate analysis were included in the multiple ordinal logistic regression analysis model for both tuberculosis patients and healthy individuals. The results showed that the level of education , vegetable intake , moderate food diversity score (DDS) of 4 - 6 were independent influencing factors of nutritional status BMI among tuberculosis patients (P < 0. 05) ; marital status was an independent influencing factor of nutritional status BMI among non⁃tuberculosis patients (P < 0. 05) ; while gender and occupation were independent influencing factors of nutritional status BMI among healthy individuals (P < 0. 05) . @*Conclusion @#The dietary nutritional status of the three population groups varied. Targeted health education should be conducted , especially for tuberculosis patients , to address the issue of uneven dietary intake and promote good dietary habits among local tuberculosis patients.
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Objective To investigate the reversal effect of hMSH2 small interference RNA(siRNA) on chemo-resistance of ovarian carcinoma cell line OC3/TAX300,explore the clinical significance.Methods The specific hMSH2 siRNA (experimental group) and non-specific hMSH2 siRNA (negative control group) was designed,synthesized and transfected into ovarian carcinoma cell line OC3/TAX300.The expression mRNA and protein levels of hMSH2 were detected by real-time reverse transcription (RT)-PCR and western blot.The cell proliferation was detected by methyl thiazolyl tetrazolium(MTT) method after 12,24,48,72 hours of 2 μg/ml taxol,the apoptosis rate after 24,48 hours of 2 μg/ml taxol was analyzed by flow cytometry.Morphological changes and ultramicrostructure of cells after 48 hours of 2 μg/ml taxol were observed with transmission electron microscope.Results (1) The mRNA levels of hMSH2 were 0.004 ± 0.000,0.053 ±0.006 and 0.057 ± 0.012 in experimental group,negative control group and non-infected group,respectively.The protein levels of hMSH2 were 0.19 ± 0.04,1.00 ± 0.07 and 0.95 ± 0.03 in experimental group,negative control group and non-infected group,respectively.(2) Compared with the noninfected group and the negative control group.The cell proliferation was effectively inhibited after 12,24,48,72 hours of 2 μg/ml taxol(P ＜0.05).The cell cycle was arrested at G2/M phase,the apoptotic rate was significantly increased after 24,48 hours of 2 μg/ml taxol (P ＜ 0.05).The experimental group after 48 hours of 2 μg/ml taxol was found to have more visible cell shrinkage,more serious chromatin margination,nucleus condensation,fragmentation and apoptotic body formation,nucleolus disappeared,markedly swollen mitochondria,mitochondrial cristae disappeared and other signs of apoptosis.While the nucleus was located in the cells of the central and nucleolus is clear,only mild chromatin pyknosis and marginalized,mild swelling of mitochondria in the control group and blank group.Conclusion siRNA targeting hMSH2 may reverse the chemo-resistance of ovarian carcinoma cell line OC3/TAX300 and may become a treatment or a new direction in the adjuvant therapy of ovarian cancer.