Asunto(s)
Carbunco/epidemiología , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/sangre , Bacillus anthracis/patogenicidad , Enfermedades de los Bovinos/epidemiología , Brotes de Enfermedades/estadística & datos numéricos , Enfermedades de los Caballos/epidemiología , Vigilancia de la Población , Animales , Carbunco/sangre , Carbunco/diagnóstico , Carbunco/inmunología , Carbunco/microbiología , Anticuerpos Antibacterianos/inmunología , Antígenos Bacterianos/inmunología , Bovinos , Enfermedades de los Bovinos/sangre , Enfermedades de los Bovinos/diagnóstico , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/microbiología , Diagnóstico Tardío , Brotes de Enfermedades/prevención & control , Reservorios de Enfermedades/microbiología , Reservorios de Enfermedades/estadística & datos numéricos , Enfermedades de los Caballos/sangre , Enfermedades de los Caballos/diagnóstico , Enfermedades de los Caballos/inmunología , Enfermedades de los Caballos/microbiología , Caballos , Humanos , Kazajstán/epidemiología , Masculino , Reacción en Cadena en Tiempo Real de la Polimerasa , RoedoresRESUMEN
Y. pestis high repeated sequences DNA (HRS) used as probes in the blot hybridization procedure made it possible to reveal some tendencies of their location on the chromosome, namely, the correlation with the regions where Y. pestis strains were isolated, correlation with the stability of their properties and, perhaps, with the variation of individual strains.
Asunto(s)
ADN Bacteriano/genética , Secuencias Repetitivas de Ácidos Nucleicos , Yersinia pestis/genética , Dermatoglifia del ADNRESUMEN
The work deals with the results obtained in the study of the activity of the cytocidal systems of phagocytes in great gerbils under normal conditions and during the plague infectious process. The data have been analyzed in comparison with the corresponding characteristics of phagocytic activity in mice. Changes in the oxygen-dependent metabolism (ODM) of neutrophils and macrophages, responsible for the functioning of their oxygen-dependent cytocidal systems, in plague show features characteristic of the nonspecific systemic postaggression reaction (SPAR). In Y. pestis-sensitive animals (mice), changes in the ODM activity of phagocytes are manifested as shock of different severity. In great gerbils, these changes present as true SPAR. The degree of the sensitivity of animals to Y. pestis infection is mainly determined by the initial level of the activity of oxygen-sensitive cytocidal systems of neutrophils (determined in the nitro blue tetrazolium spontaneous reduction test). In great gerbils this level exceeds the corresponding characteristics in mice 2.6- to 8.7-fold.
Asunto(s)
Gerbillinae/inmunología , Fagocitos/inmunología , Peste/inmunología , Animales , Inmunidad Innata , Macrófagos/inmunología , Macrófagos/metabolismo , Ratones , Neutrófilos/inmunología , Neutrófilos/metabolismo , Consumo de Oxígeno , Fagocitos/metabolismo , Fagocitosis , Peste/metabolismo , Factores de TiempoRESUMEN
Immunochemical analysis of 2 polysaccharide-containing structures of the lypopolysaccharide of the plague causative agent (main somatic antigen and lipopolysaccharide) isolated from K-1 strain and a number of its antibiotic resistant mutants was carried out. It was shown that development of resistance to streptomycin alone or its combination with monomycin did not cause detectable changes in the monosaccharide composition and serological properties of the cultures tested. More significant changes associated with development of complex resistance, i.e. K-1 (Strr leads to Penr leads to Tetr) were accompanied by a decrease in the content of hexozamine and serological activity of the main somatic antigen determining the O-specificity of the lipopolysaccharide. Defective changes in the monosaccharide composition and serological properties of both the main somatic antigen and the polysaccharide were observed in the yellow variant of the streptomycin resistant mutant K-1.
Asunto(s)
Antibacterianos/farmacología , Antígenos Bacterianos/aislamiento & purificación , Mutación/efectos de los fármacos , Polisacáridos Bacterianos/aislamiento & purificación , Yersinia pestis/inmunología , Farmacorresistencia Microbiana , Monosacáridos/análisis , Polisacáridos Bacterianos/inmunología , Yersinia pestis/efectos de los fármacosRESUMEN
By the character of the sensitivity of UV-irradiation, to N-methyl-N'-nitro-N-nitrosoguanidine, 5-bromuracil, mitomycin C, crystalviolet, and by the capacity to restore phage injuries the 1435-A and 1435-24 mutants were referred to the uvr-hcr-, 17 mutant--to the uvr-hcr+, and 35 mutant--to lon genotype. As a result of UV irradiation the experimental strains formed heteromorphic forms of bacteria, spindle-shaped, filamentous cells, were sensitive to the action of static electrical field of high frequency, this pointing to disturbance of cellular membranes structure in these mutants.