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BACKGROUND: Sharps injury constitutes one of the major occupational hazards in dental practice with practitioners under training being the most exposed group. This study aimed to assess the level of awareness about sharps injury, its prevalence, and reporting rates among dental students, trainees, and assistants. METHODS: The study was conducted at the Dental Teaching Hospital, Umm Al Qura University, Saudi Arabia, through an online self-designed questionnaire which comprised 21 items. Data was collected, tabulated, and statistically analyzed. Results: Among 182 responding participants, the mean awareness score was satisfactory in 117 participants (64.3%) and average in 64 (35.2%). Exposure to sharps injury in the last 24 months was reported by 31.3% (n=20) with needle stick injury being the most frequent cause and only 59.6% (n=34) reported the injury. Interns and sixth-year students were the most injured participants. There was a significant difference in knowledge between exposed and non-exposed participants regarding the safe technique of recapping needles (p=0.037). After the injury, 77.2% (n=44) of participants washed their hands with soap and water. CONCLUSIONS: A considerable percentage of study participants have average to satisfactory awareness about the risk of sharps injury with a high under-reporting rate. So, comprehensive preclinical education and training must be provided to our hospital's students, trainees, and assistants to increase awareness about potentially risky behavior. More orientation about reporting and its role in prevention is highly recommended to ensure safe practice and improve the quality of dental care.
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Objectives: The aim of this study is to investigate and compare the microbial efficacy of Moringa oleifera leaf extract, octenidine dihydrochloride (OCT), NaOCl, and their combinations as intracanal irrigants against Enterococcus faecalis. Materials and Methods: Sixty single-rooted mandibular premolars were decoronated followed by root canal preparation. Each root specimen was autoclaved, inoculated with E. faecalis, and incubated at 37°C for 48 hr. Then, the specimens were divided into six groups based on the irrigation solution used: 2.5% NaOCl (Group 1), 0.1% OCT (Group 2), M. oleifera leaves extract (Group 3), a combination of M. oleifera extract and 1.25% NaOCl (Group 4), a combination of M. oleifera extract and OCT (Group 5) and normal saline (Group 6). Microbial samples were taken from each root canal before (S1) and after (S2) irrigation and the bacterial viability was assessed using colony-forming units (CFU) on bile esculin agar plates. Results: Comparing the number of CFU/ml before and after irrigation showed a significant reduction (P < 0.001) in all studied groups. Comparison between the CFU/ml after irrigation by NaOCl and each of the combination groups showed a significant difference. Conclusion: M. oleifera leaves extract and 0.1% OCT solutions have antibacterial effect against E. faecalis comparable to 2.5% NaOCl and might be used as root canal irrigants. The combination groups showed better antimicrobial activities than individual irrigants. However, further studies are required to investigate the biocompatibility and possible toxic effects of the tested irrigants.
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OBJECTIVES: This study aimed to assess hepatitis B surface antibody (anti-HBs) persistence among dental students two decades after infant vaccination and immune response after revaccination or booster dose in nonimmune groups. MATERIALS AND METHODS: A cross-sectional study was performed using laboratory reports for anti-HBs performed to dental students at the Umm al-Qura University from 2016 to 2020. Reports were classified according to baseline antibody titer into group I (titer <10 mIU/mL), group II (titer 10 to <100 mIU/mL), and group III (titer ≥100 mIU/mL). The basal antibody titer of each group was correlated to student's gender and birth year and compared with postrevaccination or booster dose titer in groups I and II. STATISTICAL ANALYSIS: Data of baseline and postrevaccination anti-HBs antibody titers were analyzed using Statistical Package for Social Science (SPSS). One-way ANOVA was used to compare between different means for antibody titers of students as well as baseline and postrevaccination antibody levels for nonimmune students with nonprotective antibody titers. Pairwise multiple comparison of the difference between baseline and postrevaccination anti-HBs antibody titers were done by post hoc Tukey's honestly significant difference (HSD) test. Chi-squared test was used for comparing between the frequencies. A p-Value of ≤0.05 was considered significant and p < 0.01 was considered highly significant. RESULTS: A significant percentage (73.6%) of students had antibody titer <10 mIU/mL, while only 4.8% had antibody titer ≥100 mIU/mL with nonsignificant difference between both genders (χ 2 =3.784, p = 0.151). A statistically nonsignificant difference was also found between the three categories of anti-HBs antibody titers among students with different birth dates (χ 2 = 13.817, p = 0.182). After revaccination of nonimmune students, 100% of them showed strongly protective antibody titers with mean of 842.88 to 844.58 mIU/mL. A highly significant difference was observed between the mean baseline and postrevaccination antibody titers in both genders, with a higher mean of post revaccination (p = 0.000). CONCLUSION: Two decades after infant immunization, a significant percentage of dental students failed to maintain the anti-HBs seroprotective titer. So titer measurement should be made compulsory before they begin their clinical training and revaccination or booster dose should be given to nonimmune students to maintain a high protection level.
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OBJECTIVES: The aim of the study was to share our experience of the development and application of a modified infection control protocol at the Dental Teaching Hospital, Umm Al-Qura University (UQUDENT) during the second wave of the COVID-19 pandemic. A second aim was to evaluate the impact of the implemented strategies on preparations for future requirements in clinical dental education. MATERIALS AND METHODS: In this descriptive study, we evaluated the challenges facing dental practice and categorized them into four domains: challenges facing dental practice during the pandemic, the risk of acquiring COVID-19 infection, the design of student clinics, and the financial challenges. The impact of strategies established to deal with such challenges was studied by comparing the number of treated patients from September 1, 2020, to March 01, 2021, with the number treated during the same months pre-COVID-19. The COVID-19 polymerase chain reaction (PCR) confirmed students and health care workers (HCWs) were expressed in numbers and percentages in each category of the study group. RESULTS: Policies were set up to deal with the challenges in each domain, after training all the hospital personnel in mitigation of the spread of infection within the hospital. We left a non-working clinic between every two operating clinics, and the patient risk was assessed by triage scoring and health status checks by a Saudi mobile application at the entrance. The hospital delivered more personal protective equipment and obligated all students and HCWs to wear KN95 or N95 masks during procedures. Over 1,500 patients were treated during the study period with more than a 30% reduction in comparison to those in the pre-COVID-19 period, but only 20 UQUDENT personnel had confirmed COVID-19 infection, and all proved to be community-acquired by contact tracing. CONCLUSION: The measures implemented in this study proved effective. With the challenges and limited resources, UQUDENT managed to resume the operation of its dental clinics and training while preventing cross-infection, and it ensured that dental students graduated with the required competency. Sharing experiences between educational institutes will help to graduate safe competent practitioners.
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INTRODUCTION: Salvadora persica L. (S. persica, Siwak) has been used for many centuries as oral hygiene tools, particularly in Saudi Arabia. This study aimed to assess the effectiveness of S. persica petroleum ether extract (SPE) as an intracanal bactericidal for endodontic treatment against Enterococcus faecalis. Calcium hydroxide Ca(OH)2 gold standard intracanal medicament was used for comparison. METHODS: The gas chromatography mass spectrometry (GC/MS) analysis was carried out to identify the components of SPE. First, the consistency of SPE was accomplished according to ANSI/ADA specification no 57. Forty-five single-rooted mandibular premolars were infected with that of E. faecalis suspension. Colony-forming units (CFU) were counted before the medicaments' application (CFU-1) and after seven days of their applications (CFU-2). Group I: SPE, Group II: positive control Ca(OH)2, and Group III: saline solution negative control. The microdilution method was applied to determine minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SPE. RESULTS: Thirty-two compounds were identified (89.09%), with main components of benzyl isothiocyanate (BITC) (33.32%) and steroids (34%). CFU before and after using SPE and Ca(OH)2 recorded a statistically significant reduction in bacterial count (P=0.006) and (P=0.01), respectively. There was an insignificant difference between CFU after using SPE and Ca(OH)2 (P=0.210). On the contrary, comparing both medicaments with the negative control saline group resulted in significant differences, (P=0.001) and (P=0.007), respectively. Moreover, the equality of minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of SPE is recorded. CONCLUSION: This finding could be referred to the high content of bactericidal BITC in synergism with other antimicrobial components, representing 70.71% of SPE. Thus, SPE is a good candidate as an intracanal medicament, which warrants further investigation.
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BACKGROUND: Infection 'is a common complication in children with hematological malignancies' during febrile neutropenia. ' OBJECTIVE: The aim of this study was to' evaluate common bacterial and fungal pathogens in children with hematological malignancies during febrile neutropenia in single center Egyptian study. ' PATIENTS AND METHODS: This study was carried out on 90' children with hematological malignancies during febrile neutropenia including 54 with ALL, 27 with AML and 9 with NHL with their ages ranging from 2.5- 13 years and mean age value of 5.5 ± 3.5. Complete blood count, BM aspiration, and blood and throat cultures were done for all patients. RESULTS: Positive bacterial growth was found in 54 cultures (30%) including 42 blood cultures and 12 throat cultures with significantly higher Gram negative bacterial growth. Staphylococcus aurous and Pseudomonas aeruginosa were the most common Gram positive and negative organisms respectively. Cefepime was the most effective antibiotic against isolated bacterial growth where 80% of Gram negative bacterial growth was sensitive to it, 20% showed intermediate response and no Gram negative Cefepime resistance was recorded. On the other hand, 62.5% of Gram positive organisms was sensitive to it, 25% showed intermediate response and Gram positive Cefepime resistance was found in 12.5%. Ampicillin sulbactam 'was the most effective antibiotic against Gram positive' organisms with 100% sensitivity. Positive fungal growth was found in 36 cultures (20%) including 30 throat cultures and 6 blood cultures and all fungal isolates were candida. Amphotericin was active against 100% of fungal isolates, while resistance to Fluconazole and Voriconazole was found in 25% and 33.33% respectively. CONCLUSIONS: 'Gram negative is still more common than gram positive' infections and fungal infection is also a common cause of fever in patients with hematological malignancies during neutropenia and must be taken in consideration in every case of febrile neutropenia.
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Neutropenia Febril/microbiología , Hongos/aislamiento & purificación , Bacterias Gramnegativas/aislamiento & purificación , Neoplasias Hematológicas/microbiología , Adolescente , Ampicilina/farmacología , Antibacterianos/farmacología , Antifúngicos/farmacología , Bacteriemia , Recuento de Células Sanguíneas , Candida/efectos de los fármacos , Candida/aislamiento & purificación , Cefepima , Cefalosporinas/farmacología , Niño , Preescolar , Egipto/epidemiología , Neutropenia Febril/epidemiología , Femenino , Hongos/efectos de los fármacos , Hongos/crecimiento & desarrollo , Hongos/patogenicidad , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Gramnegativas/crecimiento & desarrollo , Bacterias Gramnegativas/patogenicidad , Humanos , Masculino , Faringe/microbiología , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/aislamiento & purificación , Pseudomonas aeruginosa/patogenicidad , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/aislamiento & purificación , Staphylococcus aureus/patogenicidadRESUMEN
BACKGROUND: Recent studies have highlighted the possible influence of chemokines and cytokines on several types of pulmonary arterial hypertension (PAH). Increasing interest has also been focussed on their role as a cause of post-cardiopulmonary bypass (CPB) organ dysfunction. HYPOTHESIS: Chemokines and cytokines are involved in the pathobiology of rheumatic PAH. METHODS: Serum levels of the chemokine, regulated upon activation, normal T-cell expressed and secreted (RANTES) and the cytokine interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA) in 35 patients with rheumatic mitral valve disease and 10 matched healthy subjects (control group). Eleven patients (31.4%) had severe pulmonary hypertension. Subsequently, 23 patients underwent mitral valve replacement. The relation of RANTES and IL-6 circulating level with postoperative organ dysfunction was analysed through multiple organ dysfunction score (MODS). RESULTS: Patients with severe PAH have a significantly higher mean serum level of RANTES compared with other patients (6138.6+/-3543.8 pg/ml vs 1818.2+/-475.2 pg/ml, p=0.0003). The serum level of IL-6 in the patients was statistically different from that of the control (378+/-50.8 pg/ml vs 262+/-90.5 pg/ml, respectively, p=0.002). Patients who required postoperative inotropes had higher preoperative and post-CPB levels of both RANTES and IL-6. While patients with postoperative lung dysfunction had higher levels of IL-6 preoperatively and post-CPB and lower levels of RANTES post-CPB. CONCLUSIONS: RANTES and IL-6 should be investigated as potential therapeutic targets in the control of rheumatic PAH. Improved understanding of the contribution of RANTES and IL-6 to adverse postoperative complications can lead to improved patient outcome.
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Quimiocina CCL5/sangre , Hipertensión Pulmonar/inmunología , Interleucina-6/sangre , Cardiopatía Reumática/inmunología , Adolescente , Adulto , Biomarcadores/sangre , Estudios de Casos y Controles , Femenino , Implantación de Prótesis de Válvulas Cardíacas/efectos adversos , Humanos , Hipertensión Pulmonar/etiología , Masculino , Persona de Mediana Edad , Insuficiencia de la Válvula Mitral/complicaciones , Insuficiencia de la Válvula Mitral/inmunología , Insuficiencia de la Válvula Mitral/cirugía , Estenosis de la Válvula Mitral/complicaciones , Estenosis de la Válvula Mitral/inmunología , Estenosis de la Válvula Mitral/cirugía , Insuficiencia Multiorgánica/etiología , Insuficiencia Multiorgánica/inmunología , Estudios Prospectivos , Cardiopatía Reumática/complicaciones , Cardiopatía Reumática/cirugía , Adulto JovenRESUMEN
We investigated the feasibility of profiling and measuring the concentration of clusterin in urine and serum for individuals with transitional cell carcinoma (TCC) of the bladder and comparing it with nontumor controls. In addition, we analyzed the correlation of expression of clusterin in specimens of TCC to various clinicopathologic parameters and prognosis of bladder cancer. Blood and urine samples were used from 68 patients with TCC of the bladder and from 61 patients with benign urological diseases. Enzyme-linked immunosorbent assays (ELISA) were performed for clusterin from serum and urine. Quantitation of clusterin mRNA was carried out in 68 bladder tumor specimens from radical cystectomy or transurethral resection and 26 normal bladder specimens from BPH patients by using RT-PCR method. Correlation for the expression of clusterin mRNA with clinicopathologic parameters was analyzed. Serum and urine clusterin was significantly higher in individuals with bladder cancer than control (p = 0.001). Sensitivity and specificity of serum and urine clusterin as a tumor marker for TCC of the bladder was found to be 80%, 91%, 87.1% and 96.7% respectively. Clusterin expression was significantly higher in TCC specimens than normal tissue specimens (P < 0.001). Expression of clusterin was significantly higher in patients with invasive TCC of the bladder than that in patients with superficial TCC and control (P < 0.001). Overexpression of clusterin mRNA was significantly associated with tumor recurrence and overall survival (p < 0.001). The recurrence-free survival time of patients with overexpression of clusterin was significantly shorter than that of patients with weak expression of clusterin (9.8 months vs. 35.2 months). Clusterin may be considered as a potential diagnostic and prognostic biomarker for bladder cancer using urine, serum and/or molecular biology techniques.