RESUMEN
Drought stress triggers sugar accumulation in plants, providing energy and aiding in protection against oxidative damage. Plant hardening under mild stress conditions has been shown to enhance plant resistance to severe stress conditions. While sugar accumulation and metabolism under drought stress have been well-documented in crop plants, the effect of drought acclimation treatment on sugar accumulation and metabolism has not yet been explored. In this study, we investigated the impact of drought stress acclimation on sugar accumulation and metabolism in the leaves and root tissues of two commonly cultivated foxtail millet (Setaria italica L.) genotypes, 'PI 689680' and 'PI 662292'. Quantification of total sugars (soluble sugar, fructose, glucose, and sucrose), their related enzymes (SPS, SuSy, NI, and AI), and the regulation of their related transcripts (SiSPS1, SiSuSy1, SiSWEET6, SiA-INV, and SiC-INV) revealed that drought-acclimated (DA) plants exhibited levels of these indicators comparable to those of control plants. However, under subsequent drought stress conditions, both the leaves and roots of non-acclimated plants accumulated higher levels of total sugars, displayed increased activity of sugar metabolism enzymes, and showed elevated expression of sugar metabolism-related transcripts compared to drought-acclimated plants. Thus, acclimation-induced restriction of sugar accumulation, transport, and metabolism could be one of the metabolic processes contributing to enhanced drought tolerance in millet. This study advocates for the use of acclimation as an effective strategy to mitigate the negative impacts of drought-induced metabolic disturbances in millet, thereby enhancing global food security and promoting sustainable agricultural systems.
RESUMEN
The impact of climate change-induced drought stress on global food security and environmental sustainability is a serious concern. While previous research has highlighted the potential benefits of drought hardening in improving plants' ability to withstand drought, the exact underlying physiological mechanisms in millet plants (Setaria italica L.) have not been explored. This study aimed to investigate the impact of drought hardening on antioxidant defense and polyphenol accumulation in different millet genotypes ('PI 689680' and 'PI 662292') subjected to different treatments: control (unstressed), drought acclimation (two stress episodes with recovery), and non-acclimation (single stress episode with no recovery). The results showed that drought stress led to higher levels of polyphenols and oxidative damage, as indicated by increased phenolic, flavonoid, and anthocyanin levels. Non-acclimated (NA) plants experienced more severe oxidative damage and inhibition of enzymes associated with the ascorbate glutathione cycle compared to drought-acclimated plants. NA plants also exhibited a significant reduction in photosynthesis and tissue water content. The expression of genes related to antioxidants and polyphenol synthesis was more pronounced in non-acclimated plants. The study demonstrated that drought hardening not only prepared plants for subsequent drought stress but also mitigated damage caused by oxidative stress in plant physiology. Drought-acclimated (DA) plants displayed improved drought tolerance, as evidenced by better growth, photosynthesis, antioxidant defense, polyphenol accumulation, and gene expression related to antioxidants and polyphenol synthesis. In conclusion, the research advocates for the use of drought hardening as an effective strategy to alleviate the negative impacts of drought-induced metabolic disturbances in millet. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01366-w.