RESUMEN
AIM: This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells. MATERIALS AND METHODS: Blood samples were taken from the jugular vein; and the smears were confectioned on slides immediately after the blood collection and stained according to the May-Gründwald Giemsa method. For the morphometric study, three parameters were considered which are: The diameter, the circumference, and the surface of erythrocytes; and measurements were achieved using the OPTIKATM Vision Pro software. Statistical analysis was performed by both analysis of variances and Student's t analytical tests. RESULTS: The recorded data showed that the three morphometric parameters of red blood cells are higher in dogs followed, respectively, by those of horses, cattle, and sheep, whereas, the goats have the lowest ones. In addition, the obtained results allowed us to propose new reference values for the circumference and the surface of erythrocyte in considered species. CONCLUSION: This investigation permit concluding that from a drop of blood it is possible to characterize the different animal species, taking into account the diameter, the circumference, and the surface of erythrocytes.
RESUMEN
This study was carried out on five cattle groups, local, cross, Prim'Holstein, Montbeliard, and Brown of the Alps, in order to study the influence of breed on erythrocytes diameter. For each breed, blood samples were taken from 15 adult females by jugular venipuncture; blood smears were made on slides immediately after the blood collection and stained according to the method of May-Gründwald Giemsa. Morphometric study was realized using the OPTIKA Pro Vision software. The statistical analysis was assessed by using the descriptive boxplots test and ANOVA. The size of red blood cells is greater in the imported Brown of the Alps breed (5,32 ± 0,19) and also in our local breed (5,23 ± 0,10), whereas they were smaller in the Montbeliard breed (4,79 ± 0,21). This investigation allowed us to show that from a drop of blood we can have an idea of the bovine breeds, taking into account the size of the erythrocytes.
RESUMEN
Camel trypanosomosis is a life-threatening disease in the camel species and responsible for severe economic losses either in milk or meat productions. This study was carried out on the south-east area of Algeria on 100 camels of various ages and either sex from two herds. Microscopic examination of blood smears revealed higher levels of trypanosomosis caused by Trypanosoma evansi, an elongated parasite with a kinetoplast and a single nucleus located in its half-length and one flagellum with great heterogeneity. This first investigation reveals higher infection rate than those observed in other countries using blood smears, the trypanosomosis attack has reached an alarming level and the occurrence of trypanosomosis at this high level on blood smears is like "the tree that hides the forest" and make up a serious and potential danger both on animal and public health. Therefore, radical preventive and offensive drastic measures must be taken against this menacing disease at the critical points to prevent the economic losses and to avoid possible human transmission.