RESUMEN
A total of 179 cases of lymphoma, both nodal and extranodal, recorded in the Papua New Guinea Tumor Registry were reviewed and classified by the updated Kiel classification. Fifteen (8.4%) were Hodgkin's disease, 39 (21.8%) probable Burkitt's lymphoma (poorly preserved), 46 (25.7%) Burkitt's lymphoma, 33 (18.4%) Burkitt's-like lymphoma, 36 (20.1%) other B-cell subtypes and 10 (5.6%) T-cell lymphoma. No case of follicular B-cell lymphoma was encountered.
Asunto(s)
Linfoma/patología , Humanos , Inmunohistoquímica , Linfoma/química , Linfoma/epidemiología , Linfoma de Células B/química , Linfoma de Células B/epidemiología , Linfoma de Células B/patología , Linfoma de Células T/química , Linfoma de Células T/epidemiología , Linfoma de Células T/patología , Papúa Nueva Guinea/epidemiologíaRESUMEN
Collagenase digestion allows cells to be released into suspension from bone marrow tissue. Discrete abnormal populations of lymphoid cells can be identified by cell morphology and immunological phenotyping techniques. Viable cells are also available for chromosomal analysis. This technique makes cells available for analysis in cases of dry bone marrow taps and has a particular use in the investigation of bone marrow involvement by malignant lymphoma.
RESUMEN
A technique for the extraction of cells from bone marrow trephine core biopsy specimens using collagenase digestion was assessed in 39 cases (33 diagnostic and six normal). Diagnostically useful numbers of cells were extracted from all marrows. Morphological assessment of cytocentrifuge preparations of these cells gave a correct diagnosis in 23 (60%) of cases compared with 27 (70%) for the corresponding aspirated marrow smears. Phenotypic analysis using flow cytometry showed persistence of a range of surface membrane antigens following collagenase digestion. Increased autofluorescence was a problem in some cases. Cytochemistry, bone marrow culture, and cytogenetic analysis could also be carried out on these cells. It is concluded that this technique has useful diagnostic applications in cases of dry taps.
Asunto(s)
Médula Ósea/patología , Colagenasa Microbiana/metabolismo , Adulto , Biopsia , Células de la Médula Ósea , ADN/análisis , Histocitoquímica , Humanos , Cariotipificación , FenotipoRESUMEN
Fatal spontaneous rupture of the liver is described in a 46-year-old women with hepatic amyloidosis diagnosed premortem by liver biopsy. An autopsy revealed systemic amyloidosis, with no evidence of an underlying chronic inflammatory condition, infection, or immunocyte dyscrasia. By computerized search, this is only the third reported case of spontaneous rupture of the liver complicating amyloidosis.