RESUMEN
3-Bromopyruvate (3BP), known for its potent anticancer properties, also exhibits remarkable efficacy against the pathogenic fungus Cryptococcus neoformans. So far it has been proven that the main fungicidal activity of 3BP is based on ATP depletion and a reduction of intracellular level of glutathione. The presented study includes a broad range of methods to further investigate the mechanistic effects of 3BP on C. neoformans cells. The use of flow cytometry allowed a thorough examination of their survival during 3BP treatment, while observations using electron microscopy made it possible to note the changes in cellular morphology. Utilizing ruthenium red, the study suggests a mitochondrial pathway may initiate programmed cell death in response to 3BP. Analysis of free radical generation and gene expression changes supports this hypothesis. These findings enhance comprehension of 3BP's mechanisms in fungal cells, paving the way for its potential application as a therapeutic agent against cryptococcosis.
Asunto(s)
Criptococosis , Cryptococcus neoformans , Cryptococcus neoformans/metabolismo , Piruvatos/metabolismo , Piruvatos/farmacología , Piruvatos/uso terapéutico , Criptococosis/tratamiento farmacológico , ApoptosisRESUMEN
Helicobacter pylori is one of the most frequent human pathogens and a leading etiological agent of various gastric diseases. As stringent response, coordinated by a SpoT protein, seems to be crucial for the survivability of H. pylori, the main goal of this article was to use in silico computational studies to find phytochemical compounds capable of binding to the active site of SpoT from H. pylori and confirm the ability of the most active candidates to interfere with the virulence of this bacterium through in vitro experiments. From 791 natural substances submitted for the virtual screening procedure, 10 were chosen and followed for further in vitro examinations. Among these, dioscin showed the most interesting parameters (the lowest MIC, the highest anti-biofilm activity in static conditions, and a relatively low stimulation of morphological transition into coccoids). Therefore, in the last part, we extended the research with a number of further experiments and observed the ability of dioscin to significantly reduce the formation of H. pylori biofilm under Bioflux-generated flow conditions and its capacity for additive enhancement of the antibacterial activity of all three commonly used antibiotics (clarithromycin, metronidazole, and levofloxacin). Based on these results, we suggest that dioscin may be an interesting candidate for new therapies targeting H. pylori survivability and virulence.
Asunto(s)
Antibacterianos/química , Productos Biológicos/química , Diosgenina/análogos & derivados , Infecciones por Helicobacter/tratamiento farmacológico , Helicobacter pylori/efectos de los fármacos , Pirofosfatasas/química , Virulencia/efectos de los fármacos , Secuencia de Aminoácidos , Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Dominio Catalítico , Claritromicina/farmacología , Diosgenina/química , Diosgenina/farmacología , Evaluación Preclínica de Medicamentos , Humanos , Levofloxacino/farmacología , Metronidazol/farmacología , Pruebas de Sensibilidad Microbiana , Simulación del Acoplamiento Molecular , Unión Proteica , Conformación ProteicaRESUMEN
Aim: Characterization of the ability of Fusobacterium nucleatum DSM 15643 and DSM 20482 strains in the presence of Cu2+ and H2O2 to reactive oxygen species generation. Method: Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Results: Determination of: MIC for Cu2+, H2O2 and ABTS; survivability of F. nucleatum under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria. Conclusion:F. nucleatum in the presence of Cu2+ and H2O2 is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.
Asunto(s)
Fusobacterium nucleatum/química , Fusobacterium nucleatum/metabolismo , Especies Reactivas de Oxígeno/análisis , Especies Reactivas de Oxígeno/metabolismo , Espectrofotometría/métodos , Benzotiazoles/química , Cobre/farmacología , Fusobacterium nucleatum/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Ácidos Sulfónicos/químicaRESUMEN
The control and prevention of biofilm-related infections is an important public healthcare issue. Given the increasing antibiotic resistance among bacteria and fungi that cause serious infections in humans, promotion of new strategies combating microorganisms has been essential. One attractive approach to inactivate microorganisms is the use of semiconductor photo-catalysis, which has become the subject of extensive research. In this study, the bactericidal properties of four photo-catalysts, TiO2, TiO2-S, TiO2-Eu and TiO2-Eu-S, were investigated against established 24, 48, 72 and 96 h biofilms of Enterococcus The exposure of biofilms to the catalysts induced the production of superoxide radical anions. The best photo-catalytic inactivation was achieved with the TiO2-Eu-S and TiO2-S nanopowders and 24 h biofilms. Transmission electron microscopy images showed significant changes in the structure of the biofilm cells following photo-inactivation. The results suggest that doping with europium and modifying the surface with sulphate groups enhanced the bactericidal activity of the TiO2 nanoparticles against enterococcal biofilms.
Asunto(s)
Biopelículas/efectos de los fármacos , Enterococcus/efectos de los fármacos , Viabilidad Microbiana , Nanopartículas/química , Fármacos Fotosensibilizantes/farmacología , Titanio/farmacología , Antibacterianos/farmacología , Biopelículas/crecimiento & desarrollo , Catálisis , Enterococcus/ultraestructura , Europio/química , Europio/farmacología , Sustancias Luminiscentes/química , Sustancias Luminiscentes/farmacología , Microscopía Electrónica de Transmisión , Nanopartículas/ultraestructura , Sulfatos/química , Superóxidos/metabolismoRESUMEN
We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with QAS, and confirmed destruction of the mutant cytoplasm, an increase in the thickness of the cell wall, separation of the cell wall from the cytoplasm, and the accumulation of numerous lipid droplets. We also observed a relatively high production of lipids in the cells of the parental wild-type strain Sigma1278b and in its IM-resistant (IM(R)) mutant in the presence of the QAS. The IM(R) mutant showed increased sensitivity to CaCl(2) and SDS, and resistance to ethidium bromide, chloramphenicol, erythromycin and osmotic shock. It also tolerated growth at low pH. We suggest that the resistance to IM could be connected with the level of permeability of the cell membrane because the IM(R) mutant was sensitive to this compound in vivo in the presence of SDS and guanidine hydrochloride, which cause increased permeability of the cell plasma membrane.
Asunto(s)
Farmacorresistencia Fúngica/efectos de los fármacos , Mutación/genética , Compuestos de Amonio Cuaternario/farmacología , Saccharomyces cerevisiae/citología , Saccharomyces cerevisiae/efectos de los fármacos , Adaptación Fisiológica/efectos de los fármacos , Recuento de Colonia Microbiana , Ácidos Grasos/metabolismo , Guanidina/farmacología , Concentración de Iones de Hidrógeno/efectos de los fármacos , Metabolismo de los Lípidos/efectos de los fármacos , Presión Osmótica/efectos de los fármacos , Fenotipo , Compuestos de Amonio Cuaternario/química , Saccharomyces cerevisiae/ultraestructura , TemperaturaRESUMEN
AIM: The aim of this study was to evaluate the distal metatarsal articular angle as a key factor in choosing between the proximal closing wedge osteotomy and scarf osteotomy. METHOD: The investigation involved 40 feet: 32 females aged 13-68 in whom 24 unilateral and 8 bilateral operations had been performed from 24 to 63 months previously. RESULTS: There were statistically significant differences between groups in the postoperative hallux valgus angle and in first metatarsal shortening. We found an inverse correlation between the preoperative distal metatarsal articular angle and intermetatarsal angle improvement after proximal closing wedge osteotomy and a positive correlation after scarf osteotomy. CONCLUSION: This study confirmed the value of distal metatarsal articular angle assessment.
Asunto(s)
Hallux Valgus/cirugía , Osteotomía/métodos , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estadísticas no Paramétricas , Resultado del TratamientoRESUMEN
The lysosomotropic action of the compounds DM-11 and DMAL-12s against Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida albicans is species- and pH-dependent. At pH 6.0, DMAL-12s is less effective against S. cerevisiae and S. pombe but more effective against C. albicans than DM-11. At pH 8.0, DMAL-12s strongly inhibits the growth of S. cerevisiae but has only a marginal effect on the resistant C. albicans. S. pombe did not grow at pH 8.0. As shown by quinacrine accumulation, DM-11 causes a general intracellular acidification in all three species, while with DMAL-12s, the acidification is marginal. Morphological changes caused by DMAL-12s in S. cerevisiae affect the cell interior but not surface structures, while S. pombe cells exhibit a thickened and wrinkled cell wall, shrunken protoplast and "grainy" plasma membrane. A large number of blisters resembling lipid droplets were observed inside S. cerevisiae and S. pombe vacuoles. The high susceptibility of S. pombe cells to the action of DM-11 and DMAL-12s contrasts with the low sensitivity of S. pombe H+-ATPase to the agents. In our C. albicans isolate, DMAL 12s did not have an effect on cell morphology and appeared to be unable to penetrate the cells, especially at pH 8.0.
Asunto(s)
Alanina/análogos & derivados , Alanina/farmacología , Antifúngicos/farmacología , Levaduras/efectos de los fármacos , beta-Alanina/análogos & derivados , beta-Alanina/farmacología , Candida albicans/efectos de los fármacos , Farmacorresistencia Fúngica , Concentración de Iones de Hidrógeno , Concentración 50 Inhibidora , Lisosomas/efectos de los fármacos , ATPasas de Translocación de Protón/antagonistas & inhibidores , Levaduras/enzimología , Levaduras/ultraestructuraRESUMEN
Quaternary ammonium salts inhibited the growth of yeast especially at pH higher (pH 8) than optimal. It was postulated that compounds integrate with the cell membrane and interfere with its functions. The yeast cell ultrastructure investigated under an electron microscope confirms this hypothesis. A relatively high percentage of cells treated at pH 6 with the quaternary ammonium salt of alanine derivative (DMALM-12) at the minimal inhibitory concentration showed an irregularity in the cell shape. No such irregularity was observed in the control. Besides, in the cells treated with the drug, practically no lipid droplets were seen at all. Inside the control cells, electron-dense round bodies were clearly seen and interpreted as vacuoles. These bodies were absent in the cells treated with DMALM-12. Although the yeast cells growing at pH 8 showed a more or less normal shape, they seemed to have difficulty in budding - no fully developed buds were found in the preparations. Only some convexities of the cell wall were seen that could be the beginning of budding which stopped early after the start. Some changes in the round bodies interpreted as vacuoles were visible: they were less dense and full of granules.