RESUMEN
Lead is a heavy metal abundant in nature that causes haematological imbalances, and hepatic and renal dysfunction, and this imbalance has been linked to oxidative stress. Several reports have shown that natural products are implicated in ameliorating metal poisonings. Ginkgo biloba is a flavonoid-rich natural herbal supplement with several pharmacological properties. The present study investigated effect of Ginkgo biloba supplement (GBS) on lead-induced toxicity. Animals were given a lead dose of 25 mg/kg for 14 days orally and then given Ginkgo biloba supplements of 50 mg/kg and 100 mg/kg orally for 14 days. Animals given GBS had significantly improved haematological and rheological parameters. GBS showed a protective impact in terms of improved kidney and liver histology, anti-oxidant enzyme activity (CAT, SOD, GSH, and MDA), organ function indices, and a lower rate of erythrocyte osmotic fragility. Conclusively, Ginkgo biloba supplementation attenuated lead toxicity by normalization of haematological imbalances, and hepatic and renal dysfunction as well as maintaining erythrocyte membrane integrity.
Asunto(s)
Ginkgo biloba , Enfermedades Renales , Acetatos/efectos adversos , Animales , Antioxidantes/farmacología , Suplementos Dietéticos , Flavonoides/farmacología , Enfermedades Renales/inducido químicamente , Enfermedades Renales/tratamiento farmacológico , Enfermedades Renales/prevención & control , Plomo/farmacología , Hígado , Masculino , Extractos Vegetales/farmacología , Ratas , Ratas Wistar , Superóxido DismutasaRESUMEN
Background: A change in posture brings about a significant alteration in cardiovascular functions. The squat test has been used to study autonomic function in White Europeans but not Black Africans. The aim of this study is to determine the cardiovascular effects of postural stress in Black African non-diabetics and Type-2 diabetics. Method: Blood pressure (BP) and heart rate (HR) was measured in 40 non-diabetics and 40Type-2 diabetics in sitting, standing from sitting, squatting and standing from squatting positions Difference in BP and HR between consecutive positions was tested using 2- way mixed ANOVA. Proportions of those who showed orthostatic hypotension and hypertension were compared with Fishers exact test. Significance was set at p <0.05. mmHg Δ: BP and HR changes evoked by standing from sitting were not different, however squatting evoked greater increase in BP in diabetics(change (Δ) SBP: 5.85±9.95 vs 17.40±13.75mmHg: Δ DBP: 0.15 ± 6.89 vs 5.10 ± 7.59 mmHg:Δ MABP:2.02 ± 6.98 vs 8.63 ± 9.34 mmHg ,p <0.05) and standing from squatting evoked greater fall BP in diabetics (ΔSBP: -9.80±13.89 vs -24.35±16.03 mmHg; Δ MABP:-2.02±6.98 vs -8.63±9.34 mmHg: Δ PP: -2.28 ±15.35 vs -14.50 ±11.96 mmHg, p < 0.05) while Δ HR did not differ. A higher proportion of diabetics showed SBP and DBP orthostatic hypertension. Conclusion: Relative to the non-diabetics, diabetics showed greater BP but not HR responses to postural stress.
RESUMEN
Background Due to increasing prevalence of diabetes and associated endothelial dysfunction, this study was carried out to investigate the effects of co-administration of adrenoceptor blockers (prazosin and propranolol) and glibenclamide on plasma biomarkers of endothelial functions in diabetic rats. Methods Experiments were carried out on 35 male Wistar rats (170-200 g). They were divided into seven groups (n=5) as follows: normal control, diabetic control, diabetic + glibenclamide (GLB-5mg/kg/day), diabetic+ prazosin (PRZ-0.5 mg/kg/day), diabetic + PRZ + GLB, diabetic + propranolol (PRP-10 mg/kg/day), diabetes + PRP + GLB. Experimental diabetes was induced with streptozotocin (60 mg/kg) and drugs were administered orally for 3 weeks. Blood pressure was measured and animals were sacrificed afterwards. Blood samples were collected by cardiac puncture, and major marker of endothelial functions, nitric oxide derivatives (NOx), as well as superoxide dismutase (SOD) and malondialdehyde (MDA) were measured on the plasma. The aorta was harvested for histological examination. Data were subjected to descriptive statistics and analysed using ANOVA at α 0.05. Results There was a significant increase in levels of NOx and SOD, and a decrease in MDA level in diabetic treated groups compared to diabetic control. Mean blood pressure increased in diabetic control and diabetic + GLB group when compared with normal control, while it was mildly reduced in diabetic group treated with PRZ and PRP, and co-administered GLB. More so, Aorta histology was altered in diabetic control groups when compared with normal control and all diabetic treated groups. Conclusions Results from this study suggest that PRZ, PRP, and GLB (singly and in combined therapy) could have a restorative effect on endothelial functions in diabetes.