RESUMEN

The calcium ionophore A23187, which was used to increase the intracellular calcium concentration ([Ca2+]i), was analyzed for effects on bovine leukemia virus (BLV) expression in two BLV infected cell lines. To clarify the role of intracellular free calcium in this response, [Ca2+]i was measured during ionophore treatment with the fluorescent calcium indicator Fura-2. Elevation of intracellular calcium under these conditions caused an enhancement of BLV gp51 and p24 synthesis as well as an activation of the BLV long terminal repeat (LTR) in a dose-dependent manner. Furthermore, it was observed that elevated levels of intracellular calcium following A23187 stimulation lead to activation of NF-kappaB. Based on inhibitor studies, we hypothesize that the effect of A23187 on BLV expression appears to be mediated by PKC.

Asunto(s)

Calcio/metabolismo , Virus de la Leucemia Bovina/genética , Virus de la Leucemia Bovina/metabolismo , Proteína Quinasa C/metabolismo , 1-(5-Isoquinolinesulfonil)-2-Metilpiperazina/farmacología , Animales , Calcimicina/farmacología , Línea Celular , Inhibidores Enzimáticos/farmacología , Regulación Viral de la Expresión Génica/efectos de los fármacos , Ionóforos/farmacología , Riñón/citología , Riñón/efectos de los fármacos , Riñón/virología , Virus de la Leucemia Bovina/efectos de los fármacos , FN-kappa B/efectos de los fármacos , FN-kappa B/metabolismo , Proteína Quinasa C/efectos de los fármacos , Transducción de Señal , Estaurosporina/farmacología , Secuencias Repetidas Terminales , Transcripción Genética