RESUMEN
The present work describes the cytochemical localization of Na(+)-K(+)-ATPase in the proximal convoluted tubule cells of the gerbil Meriones crassus. By using CeCl3 as capture agent, a fine, uniform reaction product was distributed mainly across the basolateral membranes and at the apical surface. This study provides evidence that the ATPase enzyme studied is actually Na(+)-K(+)-ATPase because acetate buffer (bleaching agent) had no decalcifying effect, nor did the subsequent application of levamisole, an inhibitor of alkaline phosphatase, which can hydrolize ATP. Such findings may serve to provide insights into the regulatory role of the Na(+)-K(+)-ATPase in intra-extra cellular balance in the basolateral membranes of the proximal convoluted tubule cells of this desert animal which show thick basal laminae with their membrane-bound bodies. However, there is a lack of knowledge regarding the transport function of the assayed enzyme and these bodies.
Asunto(s)
Túbulos Renales Proximales/enzimología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Animales , Gerbillinae , Histocitoquímica , Hidrólisis , Túbulos Renales Proximales/citología , Túbulos Renales Proximales/ultraestructura , Masculino , Microscopía Electrónica , Microvellosidades/enzimología , Microvellosidades/ultraestructuraRESUMEN
The aims of the present study were to characterize by electron microscopy the morphological features of the erythrocytes of normal adult and young camels Camelus dromedarius in a renal blood vessel and in a smear. These cells are thin and elliptical, with a slight central elevation. They are slightly larger, but thinner than those reported by other authors. It is found that the renal blood vessels in adults have a thicker basal lamina with the presence of electron dense membranous bodies. These two features were described in different parts of the uriniferous tubules of the camel in previous studies. The numerical data of the haematochemical parameters that are reported here are in general similar for both adult and young camels, while they differ from those of other studies, this may have resulted from their unknown provenance and their previous dietary regime. Moreover, these parameters probably relate to the arid environment of the animal.