RESUMEN
BACKGROUND: Transducin-like enhancer of split 1 (TLE1( is a member of the TLE family of transcriptional co-repressors that control the transcription of a wide range of genes. The aim of this study was to evaluate the prognostic role of TLE1 gene expression in patients with T-cell acute lymphoblastic leukemia (T-ALL). METHOD: This study was conducted on 97 newly diagnosed T-ALL patients admitted to the Mansoura University oncology center (59 males and 38 females) with median age (33 years) in addition to 102 apparently healthy individuals served as a control group. TLE1 gene expression was measured in both patients and control groups by real time - PCR. The calculation of relative gene expression was done using the ΔΔCt method. RESULTS: TEL1 gene expression was significantly down regulated in T-ALL cases (median 2.83) as compared to controls (median 84.65) (p < 0.001). The low TEL1 gene expression was significantly associated with CNS infiltration, non-remission and higher relapse rate (p < 0.001, 0.001 and 0.023 respectively). Likewise, Low TEL1 gene expression was significantly associated with shorter OS and DFS (P= 0.012 and 0.011 respectively). Furthermore, Low TEL1 gene expression was considered as risk predictor of relapse with OR 3.636(CI.1.422-9.295) (P =0.007); and OR 0.803(CI. 0.609-0.96) (P=0.021) and independent predictor of T-ALL patient's outcome with OR 0.619 (CI. 0.44-0.872) (P=0.006). CONCLUSION: TLE1 gene expression was significantly down regulated in T-ALL cases as compared with controls. Low TLE1 expression is independent predictor of the T-ALL patient's outcome.
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Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Biomarcadores de Tumor/metabolismo , Proteínas Co-Represoras/metabolismo , Recurrencia Local de Neoplasia/patología , Leucemia-Linfoma Linfoblástico de Células T Precursoras/patología , Adulto , Anciano , Estudios de Casos y Controles , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/tratamiento farmacológico , Recurrencia Local de Neoplasia/metabolismo , Leucemia-Linfoma Linfoblástico de Células T Precursoras/tratamiento farmacológico , Leucemia-Linfoma Linfoblástico de Células T Precursoras/metabolismo , Pronóstico , Tasa de Supervivencia , Adulto JovenRESUMEN
OBJECTIVE: Sound breastfeeding (BF) knowledge among health professionals is vital for proper institutional support of BF mothers. This study aims to measure both BF knowledge and self-efficacy (SE) of pediatric nurses in supporting BF and to determine their associated factors. STUDY DESIGN: A cross-sectional study was conducted in Mansoura University Children's Hospital and Mansoura New General Hospital during the period from January to March 2017. A total of 186 nurses completed a predesigned self-administered questionnaire developed by the investigators to measure BF knowledge and SE in BF counseling. RESULTS: The overall total means of BF knowledge and SE scores were 26.8 (6.4) and 3.8 (0.6), respectively. Linear regression showed that having bachelor education, working in neonatal department, having a child (aged 2-5 years) were significant independent predictors of BF knowledge score (R 2 = 0.448, p ≤ 0.001), while BF knowledge score is the only significant independent predictor of SE in BF counseling (R 2= 0.36, p ≤ 0.001). CONCLUSION: Higher pregraduation education, working in neonatal department, having children aged 2 to 5 years are independent predictors of BF knowledge. Improving BF knowledge may improve nurses' SE in supporting BF.