RESUMEN
The analgesic properties of Epilobium angustifolium (Ea), a plant containing flavonoids with anti-inflammatory activity, have not been sufficiently studied so far. Thus, we decided to evaluate, by the classical hot plate test and the writhing test, the analgesic effect of a dry extract of Ea obtained by evaporating a commercially available mother tincture. In the former assay, the effect of Ea (380 mg/kg) was slightly lower than that of morphine (10 mg/kg s.c.). In the writhing test, which is more sensitive for non-steroidal analgesics, the effect of Ea was already significant (P < 0.05) at 95 mg/kg while at doses > or = 190 mg/kg, its activity was similar to that of lysine acetylsalicylate (300 mg/kg). The LD50 of this dry extract of Ea was 1.4+/-0.1 g/kg. Further studies are necessary for the identification of the active principles and the elucidation of their mechanism of action.
Asunto(s)
Analgésicos no Narcóticos/farmacología , Onagraceae/química , Plantas Medicinales/química , Acetatos , Analgésicos no Narcóticos/toxicidad , Animales , Relación Dosis-Respuesta a Droga , Calor , Indicadores y Reactivos , Dosificación Letal Mediana , Ratones , Dimensión del Dolor/efectos de los fármacos , Extractos Vegetales , Tiempo de Reacción/efectos de los fármacosRESUMEN
Hydrastis or goldenseal, one of the most popular medicinal herbs in the U.S.A., is used in mild pathological conditions like cold and flu, based on the pharmacological properties of its active components, berberine (anticholinergic, antisecretory, and antimicrobial) and beta-hydrastine (astringent). We previously reported the relaxant effect of a total ethanolic extract of hydrastis on carbachol precontracted isolated guinea pig trachea, and with the present study, using the same experimental model, we aimed at evaluating the contribution of its major alkaloids, berberine, beta-hydrastine, canadine and canadaline to the total effect. Furthermore, using specific pharmacological tools, like timolol and xanthine amine congener, we attempted to elucidate its mechanism of action. The EC50 of berberine, beta-hydrastine, canadine and canadaline, were 34.2+/-0.6, 72.8+/-0.6, 11.9+/-1.2 and 2.4+/-0.8 microg/ml, respectively. Timolol effectively antagonized the effect of canadine (EC50 = 19.7+/-3.0 microg/ml) and canadaline (EC50 = 17.1+/-1.2 microg/ml) but not that of berberine and beta-hydrastine, while xanthine amine congener antagonized the effect of beta-hydrastine (EC50 = 149.9+/-35.3 microg/ml) and canadaline (EC50 = 26.1+/-3.0 microg/ml) but not that of berberine and canadine. Besides, the hydrastis extract, at concentrations between 0.01 and 0.1 microg/ml, potentiated the relaxant effect of isoprenaline on carbachol-precontracted isolated guinea pig trachea. These data, which are insufficient to draw definite mechanistic conclusions, indicate that the aforementioned alkaloids may act by interacting with adrenergic and adenosinic receptors.
Asunto(s)
Alcaloides/farmacología , Berberina/análogos & derivados , Relajación Muscular/efectos de los fármacos , Músculo Liso/efectos de los fármacos , Extractos Vegetales/farmacología , Plantas Medicinales , Tráquea/efectos de los fármacos , Animales , Bencilisoquinolinas , Berberina/farmacología , Broncodilatadores/farmacología , Terapias Complementarias , Sinergismo Farmacológico , Cobayas , Técnicas In Vitro , Isoproterenol/farmacología , MasculinoRESUMEN
The mechanism responsible for the antispermatogenic activity of lonidamine (LND) [1-(2,4-dichlorobenzyl)-1H-indazole-3-carboxylic acid], a drug with low systemic toxicity and lack of significant hormonal effects, is still unclear but may be related to alterations of Sertoli cell proteins. Here, we confirmed that a single oral dose of LND (100 mg/kg b.w.) to sexually mature Sprague-Dawley rats causes shrinkage and weight reduction of the testes after 48 h. These macroscopic changes correlated with histologic alterations revealed by light microscopy, consistent with partially reversible inhibition of spermatogenesis. When the testes and the epididymides of animals treated with or without LND were homogenized and analyzed by the Bradford assay, a significant increase of total protein content was observed after 24 and 48 h. When these homogenates were analyzed by concanavalin blotting, specific changes of the major rat macroglobulins, i.e. alpha(1)-inhibitor-3, alpha(2)-macroglobulin, and alpha(1)-macroglobulin, were noted. In particular, LND caused a decrease of testicular alpha(1)-inhibitor-3, but not an increase of testicular alpha(2)-macroglobulin, indicating a mild local inflammatory response to the drug.
Asunto(s)
Antiespermatogénicos/toxicidad , Indazoles/toxicidad , Macroglobulinas/metabolismo , Testículo/efectos de los fármacos , Proteínas de Fase Aguda/metabolismo , Animales , Electroforesis en Gel de Poliacrilamida , Masculino , Orquitis/inducido químicamente , Orquitis/metabolismo , Orquitis/patología , Tamaño de los Órganos/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Espermatogénesis/efectos de los fármacos , Testículo/metabolismo , Testículo/patología , alfa-Macroglobulinas/metabolismoRESUMEN
Acute effects of aflatoxins (AF), and in particular cardiac actions, have not been examined as much as chronic toxicity. Thus, in the present study we evaluated the effects of specific AF on isolated guinea pig atria. Isoprenaline (ISO, 4x10(-9)), AFB(1) (3x10(-6) and 6x10(-5) M) and AFG(1) (3x10(-6) and 6x10(-6) M) contracted the isolated guinea pig atria, leaving the preparation hyperresponsive to ISO. These properties of AF are of interest as they could be responsible of certain cardiotoxic effects described in the literature.
Asunto(s)
Aflatoxinas/toxicidad , Contracción Miocárdica/efectos de los fármacos , Animales , Función Atrial , Cobayas , Atrios Cardíacos/efectos de los fármacos , Técnicas In Vitro , Isoproterenol/farmacología , Masculino , Estimulación QuímicaRESUMEN
Dyspnea is one of the symptoms of acute aflatoxicosis. Contrary to expectations, we observed that naturally occurring aflatoxins (AF) AFB(1), AFB(2), AFG(1), and AFG(2) and their major metabolites AFM(1), AFM(2), AFP(1), AFQ(1), and AFG(2a) relaxed carbachol (C) precontracted guinea pig trachea to different degrees. The efficacies but not the potencies of AFB(1), AFB(2), AFG(1), and AFG(2) were similar to that of the beta-agonist, isoprenaline, whose activity was potentiated by the AF. Their mechanism of action is not clearly understood but several mechanistic indications were obtained with AFB(1): 1) its effect was not influenced by the beta-blocker, timolol, indicating that a direct interaction with beta(2)-adrenergic receptors was not involved. 2) AFB(1) potentiated PGE(1) and PGE(2), two relaxant prostaglandins, and its activity was reduced by indomethacin. 3) The cAMP level in the guinea pig trachea relaxed by AFB(1) increased, possibly due to inhibition of phosphodiesterase; direct interaction with PG receptors; and/or interaction with A(2) adenosinic receptors, suggested by the inhibitory activity of XAC, a specific antagonist. 4) Finally, since tetrodotoxin reduced the relaxant activity of AFB(1), it is speculated that this mycotoxin could stimulate inhibitory nonadrenergic, noncholinergic nerves (i-NANC). In conclusion, the symptoms of acute aflatoxicosis do not seem to be due to a direct activity on the tracheal muscle, but rather, to the well-known pro-inflammatory activity of the aflatoxins, which are capable of releasing arachidonic acid from cell membranes.
Asunto(s)
Aflatoxinas/toxicidad , Carcinógenos/toxicidad , Músculo Liso/efectos de los fármacos , Tráquea/efectos de los fármacos , Animales , Broncodilatadores/farmacología , Carbacol/farmacología , AMP Cíclico/metabolismo , Cobayas , Técnicas In Vitro , Isoproterenol/farmacología , Masculino , Agonistas Muscarínicos/farmacología , Relajación Muscular/efectos de los fármacos , Músculo Liso/inervación , Prostaglandinas/fisiología , Receptores Adrenérgicos beta/efectos de los fármacos , Receptores Purinérgicos P1/efectos de los fármacos , Tetrodotoxina/farmacología , Tráquea/inervaciónRESUMEN
Acute effects of aflatoxins (AF), and in particular hormonal actions, have not been examined as much as chronic toxicity. Thus, we studied the effects of specific AF on prolactin (PRL) secretion by rat pituitary cells in culture. AFB1 and AFQ1 (1 x 10(-4) M) reduced the stimulating effect of dimethyl sulfoxide on PRL secretion by cultured rat pituitary cells. The mechanism responsible for this action is still unknown, but it did not seem to be a non specific toxic effect, because AFB1, at the same concentration, did not significantly alter cell viability, as indicated by the Trypan blue dye-exclusion test.