RESUMEN
Cancer is a threatening disease that needs strong therapy with fewer side effects. A lot of different types of chemotherapy or chemo-drugs are used in cancer treatments but have many side effects. The increasing number of antibiotic-resistant microorganisms requires more study of new antimicrobial compounds and delivery and targeting strategies. This work aims to isolate and identify Azotobacter sp., and extract alginate from Azotobacter sp. As well as fabricating selenium nanoparticles using ascorbic acid as reducing agent (As/Se-NPs), and loading extracted alginate with selenium nanoparticles (Alg-Se-NCMs). The As/Se-NPs and Alg-Se-NCMs were categorized by TEM, EDX, UV-Vis spectrophotometry, FT-IR, and zeta potential. The antifungal activities of both As/Se-NPs and Alg-Se-NCMs were investigated against some human pathogen fungi that cause skin infection such as Aspergillus niger (RCMB 002005), Aspergillus fumigatus (RCMB 002008), Cryptococcus neoformans (RCMB 0049001), Candida albicans (RCMB 005003), and Penicillium marneffei (RCMB 001002). The anticancer activities were determined against HCT-116 colorectal cancer and Hep G2 human liver cancer cells. UV spectra of As/Se-NPs and Alg-Se-NCMs confirmed a surface plasmon resonance at 269 and 296 nm, and zeta potential has negative charges -37.2 and -38.7 mV,. Both As/Se-NPs and Alg-Se-NCMs were hexagonal, size ranging from 16.52 to 97.06 and 17.29 to 44.2. Alg-Se-NCMs had anticancer activities against HCT-116 and HepG2. The Alg-Se-NCMs possessed the highest antifungal activities against Cryptococcus neoformans, followed by Aspergillus niger, but did not possess any activities against Penicillium marneffei. Alginate-capped selenium nanoparticles can be used as antifungal and anticancer treatments.
RESUMEN
BACKGROUND: Most microorganisms that cause food decay and the lower the shelf life of foods are fungi. Nanotechnologies can combat various diseases and deal with the application of nanomaterial to target cells or tissues. In this study selenium nanoparticles (Se-NPs) were synthesized using ascorbic acid and characterized by ultraviolet-visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction and zeta potential. The different concentrations of As/Se-NPs were tested against various fungi, including Alternaria linicola, Alternaria padwickii, Botrytis cinerea, Bipolaris sp., Cephalosporium acremonium, Fusarium moniliform and Fusarium semitectum. This study tested the influence of coated As/Se-NPs on healthy strawberry fruits and those infected with Botrytis cinerea during 16 days of storage, with regard to shelf life, decay percentage, weight loss, total titratable acidity percentage, total soluble solids content (TSS) and anthocyanin content. RESULTS: Energy-dispersive X-ray analysis showed only two elements: selenium and oxygen. TEM images showed that the nanoparticles ranged in size between 26 to 39 nm and were rhombohedral in shape. Se-NPs showed antifungal activity against all tested fungi, the most effective being against Botrytis cinerea, Cephalosporium acremonium and Fusarium semitectum. During storage periods of strawberries fruits coated with As/Se-NPs, the shelf life was increased, and the number of decaying fruits was less than in control (uncoated) and coated infected fruits. The decline in weight loss was lower in coated fruits than in control fruits. CONCLUSION: These findings demonstrated that As/Se-NPs could effectively maintain the postharvest quality of strawberries, even when the fruit was infected with B. cinerea. © 2024 Society of Chemical Industry.
Asunto(s)
Botrytis , Conservación de Alimentos , Fragaria , Frutas , Hongos , Fusarium , Nanopartículas , Enfermedades de las Plantas , Selenio , Fragaria/microbiología , Fragaria/química , Frutas/química , Frutas/microbiología , Conservación de Alimentos/métodos , Selenio/farmacología , Selenio/química , Botrytis/efectos de los fármacos , Botrytis/crecimiento & desarrollo , Enfermedades de las Plantas/microbiología , Enfermedades de las Plantas/prevención & control , Nanopartículas/química , Hongos/efectos de los fármacos , Fusarium/efectos de los fármacos , Fusarium/crecimiento & desarrollo , Antifúngicos/farmacología , Antifúngicos/química , Alternaria/efectos de los fármacos , Alternaria/crecimiento & desarrollo , Fungicidas Industriales/farmacología , Fungicidas Industriales/química , Almacenamiento de AlimentosRESUMEN
Lead (Pb2+) is among the most toxic heavy metals even in low concentration and cause toxicity to human's health and other forms of life. It is released into the environment through different industrial activities. The biosorption of Pb2+ from aqueous solutions by biomass of commonly available, marine alga Gelidium amansii was studied. The effects of different variables on Pb2+ removal were estimated by a two-level Plackett-Burman factorial design to determine the most significant variables affecting Pb2+ removal % from aqueous solutions. Initial pH, Pb2+ concentration and temperature were the most significant factors affecting Pb2+ removal chosen for further optimization using rotatable central composite design. The maximum removal percentage (100%) of Pb2+ from aqueous solution by Gelidium amansii biomass was found under the optimum conditions: initial Pb2+ concentration of 200 mg/L, temperature 45 °C, pH 4.5, Gelidium amansii biomass of 1 g/L and contact time of 60 minutes at static condition. FTIR analysis of algal biomass revealed the presence of carbonyl, methylene, phosphate, carbonate and phenolic groups, which are involved in the Pb2+ ions biosorption process. SEM analysis demonstrates the ability of Gelidium amansii biomass to adsorb and removes Pb2+ from aqueous solution. EDS analysis shows the additional optical absorption peak corresponding to the Pb2+ which confirms the involvement of Gelidium amansii biomass in the adsorption of Pb2+ ions from aqueous solution. Immobilized Gelidium amansii biomass was effective in Pb2+ removal (100%) from aqueous solution at an initial concentration of 200 mg/L for 3 h. In conclusion, it is demonstrated that the red marine alga Gelidium amansii biomass is a promising, efficient, ecofriendly, cost-effective and biodegradable biosorbent for the removal of Pb2+ from the environment and wastewater effluents.
Asunto(s)
Biomasa , Plomo/química , Rhodophyta/química , Contaminantes Químicos del Agua/química , Purificación del Agua/métodosRESUMEN
Cadmium is a global heavy metal pollutant. Marine green algae were used as efficient, low cost and eco-friendly biosorbent for cadmium ions removal from aqueous solutions. Plackett-Burman design was applied to determine the most significant factors for maximum cadmium removal from aqueous solutions using dry Ulva fasciata biomass. The most significant factors affecting cadmium removal process were further optimized by the face centered central composite design. The results indicated that 4 g of dry Ulva fasciata biomass was found to successfully remove 99.96% of cadmium from aqueous solution under the conditions of 200 mg/L of initial cadmium concentration at pH 5, 25 °C for 60 min of contact time with static condition. Dry Ulva fasciata biomass samples before and after cadmium biosorption were analyzed using SEM, EDS and FTIR. Furthermore, the immobilized biomass in sodium alginate-beads removed 99.98% of cadmium from aqueous solution at an initial concentration of 200 mg/L after 4 h which is significantly higher than that for control using sodium alginate beads without incorporation of the algal biomass (98.19%). Dry biomass of Ulva fasciata was proven to be cost-effective and efficient to eliminate heavy metals especially cadmium from aquatic effluents and the process is feasible, reliable and eco-friendly.