RESUMEN

A monoclonal antibody (mAb) against C. perfringens type D epsilon toxin was produced in hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA correlated with the conventional MNT for estimation of antitoxin level.

Asunto(s)

Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Monoclonales/biosíntesis , Toxinas Bacterianas/inmunología , Clostridium perfringens/inmunología , Animales , Toxinas Bacterianas/toxicidad , Vacunas Bacterianas/inmunología , Vacunas Bacterianas/farmacología , Ensayo de Inmunoadsorción Enzimática , Hibridomas/inmunología , Ratones , Ratones Endogámicos BALB C , Pruebas de Neutralización , Conejos